LOCUS       sequence-132680-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             94..159
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ggctgccgac cccgggggtg gaccatcctc tagactgccg gatctagcta gttaattaag
       61 gatccgttta gtgaaccgtc agaattaacc atggactaca aagaccatga cggtgattat
      121 aaagatcatg acatcgatta caaggatgac gatgacaagc tcatggacaa gaagtactcc
      181 attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
      241 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
      301 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
      361 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
      421 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
      481 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
      541 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
      601 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
      661 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
      721 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
      781 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
      841 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
      901 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
      961 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
     1021 gcagaccttt tt
//
