LOCUS       sequence-132681-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to 
            purify specific genomic regions of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Fujita T, Fujii H
  TITLE     Identification of Proteins Associated with an IFNgamma-Responsive 
            Promoter by a Retroviral Expression System for enChIP Using CRISPR.
  JOURNAL   PLoS One. 2014 Jul 22;9(7):e103084. doi: 
            10.1371/journal.pone.0103084. eCollection 2014.
  PUBMED    25051498
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-07-22"; volume: "9(7):e103084. doi"; pages: " 
            10.1371/journal.pone.0103084. eCollection 2014"
COMMENT     Sequence Label: 3xFLAG-dCas9/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(169..189)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgtgctggcg gccgctcgag tttaaatagt acataatgga tttccttacg cgaaatacgg
       61 gcagacatgg cctgcccggt tattattatt tttgacacca gaccaactgg taatggtagc
      121 gaccggcgct cagctggaat tcgatccggt agggatcgaa ccctttcaca ccttcctctt
      181 cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc ttgtttcata
      241 gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta
      301 ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt
      361 cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt
      421 attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa
      481 ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca cgaacagctg
      541 cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg ccagatacaa
      601 gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct cgcccgcact
      661 agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg gaagcttaat
      721 gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga tggggttttt
      781 ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc ttttgagttt
      841 tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga ctgtaggaga
      901 atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca gcttgtcgct
      961 gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt cggtctttt
//
