LOCUS       sequence-132688-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mouse targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, 
            Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM
  TITLE     Recombinase-based conditional and reversible gene regulation via XTR
            alleles.
  JOURNAL   Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783.
  PUBMED    26537451
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-5"; volume: "6:8783. doi"; pages: " 
            10.1038/ncomms9783"
COMMENT     Sequence Label: pNeoXTR f1
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     208..229
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    356..389
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    356..389
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    436..469
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    475..508
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(564..584)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(564..582)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(603..619)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(603..619)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(616..638)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    627..643
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(651..657,658..675,676..681))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    696..717
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
       61 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
      121 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
      181 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
      241 ccccggtgaa cagctcctcg cccttgctca ccatccctgg aaaaaagaaa tgcgaagatt
      301 gcgcctgcgc atcgataaaa taaaagattt tatttagtct ccagaaaaag cccggataac
      361 ttcgtataag gtatcctata cgaagttatc cattcaggct gtgctagcat caatggcatg
      421 gcacaaagct tagccataac ttcgtataat gtgtactata cgaagttatc ccgggaagtt
      481 cctattctct agaaagtata ggaacttctt aattaagacg tccttaagcc taggccaccg
      541 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
      601 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
      661 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
      721 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
      781 atcggccaac gcgcggggag aggcggtttg cgtattgg
//
