LOCUS       sequence-13269-e        7484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Gumbiner-Russo LM, Lombardo MJ, Ponder RG, Rosenberg SM
  TITLE     The TGV transgenic vectors for single-copy gene expression from the 
            Escherichia coli chromosome.
  JOURNAL   Gene. 2001 Jul 25. 273(1):97-104.
  PUBMED    11483365
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene. 2001 
            Jul 25. 273(1):97-104."
COMMENT     Sequence Label: pTGV-Express-CAT SMR5916
FEATURES             Location/Qualifiers
     source          1..7484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2112..2771)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPXXRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2686..2705
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        2905..3189
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     3112..3131
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(5505..5522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5676..6264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5756..5775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6435..7226,7227..7295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7058..7077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7296..7400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(7468..7484,1..2)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gccctggcgc gcctaggcca tatgggcccg ggcgagcata ttgatccgct gcaaactgaa
       61 attggcagca tggtctatgc ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg
      121 agctgccagc gtgtgattgg cggtctggcg aatattgccg aggagtacgc gactaaacgc
      181 tatcgttcta acgtcatcaa ctgggggatg ttaccgctgc aaatggcgga agtaccaacc
      241 tttgaagtgg gggattacat ttacatccct ggcattaaag cggcgctgga taatccgggt
      301 acgacgttta aaggttatgt gatccatgaa gatgcgccgg taacggaaat tacgctctat
      361 atggaaagtc tgactgctga agagcgcgag attatcaagg cgggtagttt gattaacttc
      421 aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt
      481 gtcggatgcg atgcttgcgc atcttatccg acctacgaat cgcatcgaat ctgtaggcca
      541 gataaggcat tttcgcagca tccggcactt attgtcggat gcgatgcttg cgcatcttat
      601 ccgacctaca aatcgcatcg aaccgtaggc cggataaggc gtttacgccg catccggcaa
      661 atagttaatt gctcttactt cttcgcctct gcaaccactt tactacccac gccgcggtta
      721 ttgtattccc acatgcggtt gtagttagtg tcattcagat tgcgctgtat ttcgtcgtta
      781 tcatctacgc tgccggtatt acccgcaaac ggacgattag agatcaccgc atcggcccac
      841 ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac ggatcaccac ctgaccgttg
      901 gtattggcat caacatccag cgagcggccc agttgcgcca caccatcacc gaaagcattg
      961 aaacggctgt ttacggcgag gaaaccgtag taaatgttgg acagcgtagc cggtgcaaac
     1021 acatacgctt cttgctgagt acgtgagttc accacgcgga attcggtgtt atcgaacacc
     1081 actgcgccgc gaccagaaac gatatccaca tccccttcaa tgtagctgtt ggtcaccagc
     1141 gtacgcggct gacgattcgt ttccagacgg ttctgcacac cgctgttggt gacaaagaag
     1201 gtgttctgac gaccgagaat gttaacgttg ttaatctgta cctggtcacc atcagtacgc
     1261 agtgccaccg ccggatggtt acctgcatct acgctatcgc ccagcgtgtt ttcgatggtc
     1321 agattttgca gttgcaggcc attgttttgt gaccagaaga ccgcagagca gagaacaccg
     1381 atactgtcgc tgcgtttgct ctggcagcta tcgtacatat accacgctgg tttacctggc
     1441 atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg cagggctcat gccaccatca
     1501 agggaaagcc caatcttcac atcaatcggt ttttcacctg taccgtacag agtaattcca
     1561 cccggagcgg cagggacata taccgttccc tgatactcac caggcatcac ggcaatatac
     1621 tggcgcttgt tggtacgctt gataattgcc gcatctaccg ccgcctgaat cgtggtatgc
     1681 gttacacctt gagtgcccgc cgggccgaca acaaagtcag gttgcgcagg cagggtaatc
     1741 ggggaaggat tccacgctgc agcacctggt gtcagggatg caaaatagtg ttgagcatcg
     1801 aaattctgcg cttcttttgc cgacagaatc gggcgagaag aggtaccagg cgcggtttga
     1861 tcagaaggac gttgatcggg cggggttgag ctacaggcgg tcagcgtcac gccaaaagcc
     1921 aatgccagcg ccagacggga aactgaaaat gtgttcacag gttgctccgg gctatgaaat
     1981 agaaaaatga atccgttgaa ggcatgtctg agctcctgtc atgcaagctt cgcagaccaa
     2041 aacgatctca agaagatcat cttattaatc agataaaata tttctaggca ccaataactg
     2101 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     2161 attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc
     2221 agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg
     2281 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     2341 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     2401 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     2461 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     2521 atcaccagct caccgtcttt cattgccata cgnnnnnncg gatgagcatt catcaggcgg
     2581 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     2641 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     2701 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     2761 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     2821 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacaagctt gcatgcatga
     2881 caggagctct cccgccattc agagaagaaa ccaattgtcc atattgcatc agacattgcc
     2941 gtcactgcgt cttttactgg ctcttctcgc taacccaacc ggtaaccccg cttattaaaa
     3001 gcattctgta acaaagcggg accaaagcca tgacaaaaac gcgtaacaaa agtgtctata
     3061 atcacggcag aaaagtccac attgattatt tgcacggcgt cacactttgc tatgccatag
     3121 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg
     3181 tttctccata cccgttttag gaggtactga gcatgcgtct agagctcgag gtagatctga
     3241 aagcttgaat cgatgtacgt actataccgg tgcgaaacgg gaaggtaaaa agacaaaaag
     3301 ttgtttttaa tacctttaag tgataccaga tggcattgcg ccatctggca gagtgattaa
     3361 ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt aaacccgacc
     3421 atcgcgccgc tggcaccttc atcgacatca atacgttcta tatccagcgc gtgaacggta
     3481 aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc catcgtaccc ggttttacca
     3541 aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga gccaaaccct
     3601 tgcggtaata cgcgggtatc agcgggtaag ttaacaacta cccagtgcca ccagccggag
     3661 ccggttggcg catccgggtc gtagcaggtg acaacaaaac ttttcgttcc cgcaggaaca
     3721 tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc gttaaagaca
     3781 tgacgatgcg gcaatttatc gccatcgcgc agatcgttac tgatgagttt catgaaccct
     3841 cctttcttgt ttgcagaaag tgtagccaga aaccctcacg cggacttctc gttattggca
     3901 aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct gcaactgttg
     3961 cgggagaata atatagggcg gcatcaggta aatcagtttg ccaaaaggcc ggatccagac
     4021 accctgttcg acaaagaatt tttgcagcgc cgccatattc accggatgag tggtttcgac
     4081 cacgccaatg gcccccagta cgcgcacatc ggcaaccatt tcggcatcac gggcgggggc
     4141 aagttgctcg cgcagctgta cttcaatatc cgccacctgt tgctgccagt cgccagattc
     4201 gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc ggattgccca taaaagttgg
     4261 cccatgcata aagcaaccgg cttcaccgtt actgatggtt tctgcaacct cgcgcgtggt
     4321 gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc acaaaatgtc
     4381 cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg gtacgaccaa atccagtggc
     4441 gatctcgtcg gcaatcagca agataccttc gcgatcgcat attttgcgga ttcgttttaa
     4501 ccattccgga tggtacatgc gcatcccgcc tgcgccctgg acaatcggct caatgatcac
     4561 cgccgcgatt tcatgacgat gcgccgccat caggcgggca aagcccacca tatcgcgctc
     4621 atcccattcg ccatccatgc ggctttgcgg ggcgggagca aacaggtttt ctggcaggta
     4681 gcctttccac agactgtgca ttgagttatc cggatcgcac accgacatcg cgccaaaggt
     4741 atcgccatga taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc ctttggcttg
     4801 ccagtactgc aacgccattt tcatcgccac ttccaccgct acggaaccgg agtccgcgag
     4861 aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt ttgcggcaca gctcaatggc
     4921 tggcgcatgg gtgataccgc caaacatcac atgcgacatg gcatcaattt gcgacttcat
     4981 cgccgcatta agctgcgggt gattgtagcc gtggatcgcc gcccaccagg acgacatacc
     5041 gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa ccttcggcgc tcaccaccgg
     5101 ataaaccggc agaggggagg tcatggatgt gtatgggtgc cagatatggc gttggtcaaa
     5161 ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat ttaggtttac
     5221 aagtctacac cgaattaaca acaaaaaaca cgttttggag aagccccatg gctcaccgcc
     5281 cacgctggac attgtcgcaa gtcacagaat tatttgaaaa accgttgctg gatctgctgt
     5341 ttgaagcgca gcaggtgcat cgccagcatt tcgatcctcg tcaggtgcag gtcagcacgt
     5401 tgctgcatat ggcgcgccta ggccaattgg gcccgggcat atgggtgtga aataccgcac
     5461 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     5521 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5581 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5641 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5701 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5761 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5821 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5881 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5941 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6001 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6061 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     6121 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6181 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6241 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6301 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     6361 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6421 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6481 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6541 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6601 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6661 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6721 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     6781 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6841 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6901 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6961 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7021 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     7081 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7141 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7201 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7261 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7321 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7381 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     7441 attattatca tgacattaac ctataaaaat aggcgtatca cgag
//
