LOCUS       sequence-132690-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible human expression vector that adds a ""flexible"" 
            3XFLAG,V5,YFP N terminus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Mita P, Lhakhang T, Li D, Eichinger DJ, Fenyo D, Boeke JD
  TITLE     Fluorescence ImmunoPrecipitation (FLIP): a Novel Assay for 
            High-Throughput IP.
  JOURNAL   Biol Proced Online. 2016 Aug 15;18:16. doi: 
            10.1186/s12575-016-0046-x. eCollection 2016.
  PUBMED    27528826
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Proced
            Online."; date: "2016-08-15"; volume: "18:16. doi"; pages: " 
            10.1186/s12575-016-0046-x. eCollection 2016"
COMMENT     Sequence Label: HuEV-A
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    62..186
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(227..532)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(329..348)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 cggccttgcc ggcctcgagc ggccgctagc aagcttgcta gcagctggta ccggatccat
       61 caccactttg tacaagaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
      121 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
      181 actatggtcg acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga
      241 acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt
      301 ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat
      361 ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc agacgtgcac
      421 tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc tgtacatcca
      481 caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc atttcaccag
      541 cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac ctcagccatc
      601 ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc attctgcatg
      661 gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac tgatagctgt
      721 cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg acatacttcg
      781 ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat acgcatactg
      841 ttatctggct tttagtaagc cggatccacg cgtttacgcc ccgccctgcc actcatcgca
      901 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
      961 aacctgaatc gccagcggca tcagcacctt gtcgcct
//
