LOCUS       sequence-132692-         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mouse targeting vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Robles-Oteiza C, Taylor S, Yates T, Cicchini M, Lauderback B, 
            Cashman CR, Burds AA, Winslow MM, Jacks T, Feldser DM
  TITLE     Recombinase-based conditional and reversible gene regulation via XTR
            alleles.
  JOURNAL   Nat Commun. 2015 Nov 5;6:8783. doi: 10.1038/ncomms9783.
  PUBMED    26537451
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-5"; volume: "6:8783. doi"; pages: " 
            10.1038/ncomms9783"
COMMENT     Sequence Label: pNeoXTR f2
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..231
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    357..390
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    357..390
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    437..470
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    476..509
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(565..585)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(565..583)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(604..620)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(617..639)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    628..644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(652..658,659..676,677..682))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    697..718
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct
       61 gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca cgggcagctt
      121 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      181 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      241 caccccggtg aacagctcct cgcccttgct caccatcctg gaaaaaagaa atgcgaagat
      301 tgcgcctgcg catcgataaa ataaaagatt ttatttagtc tccagaaaaa gcccggataa
      361 cttcgtataa ggtatcctat acgaagttat ccattcaggc tgtgctagca tcaatggcat
      421 ggcacaaagc ttagccataa cttcgtataa tgtgtactat acgaagttat cccgggaagt
      481 tcctattctc tagaaagtat aggaacttct taattaagac gtccttaagc ctaggccacc
      541 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      601 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      661 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      721 tgcgttgcgc tcactgcccg ctttcca
//
