LOCUS       sequence-132697-        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GEMIN6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  TITLE     Dreyfuss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1+ GEMIN6 myc HIS
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..61)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(645..664)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(646..664)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(709..912)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(738..758)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggtgaggt gagatgaccg gtatgcatat tcagatcctc ttctgagatg agtttttgtt
       61 cgaagggccc tctagactcg agcggccgcc actgtgctgg atatctgcag aattcttggg
      121 aagctgtaag atgtccttca ataagatcct gaacacgcga cagaataatc tcattagagc
      181 tgctgcaatt ttctggacca tatggtgggt ctatagtcag gaccccagcc acacagagag
      241 tccttggagc gtctccctgt tcagtgatgg ggatgtggtt cttctcaagc catttcttta
      301 ggctgttctt tctctcttcc agatcctctg ggctgtatgc tttgcagtct ccagacgtga
      361 acaaatgcat cagcttctcc ctcactctat ggtccccttc attcatagtt tcaacagtct
      421 gcacagcatg tcccataatt ccggtcacag acatgctgcc atcttcaagg aagttcacaa
      481 ggacaatatt ggcagagact gggtctgtag ttaaaaccca tcctttatac tcattcttct
      541 cactggctgt cactcggacc tctttgtaaa tgtaatcttg ccattctaag gggcctttct
      601 tcatccattc actcatgata agcttaacta gccagcttgg gtctccctat agtgagtcgt
      661 attaatttcg ataagccagt aagcagtggg ttctctagtt agccagagag ctctgcttat
      721 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
      781 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
      841 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
      901 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
      961 gtcccataag gtcatgtact gggcataatg ccaggcgggc canttacngt cattnacgtc
     1021 aatagggggc gtacttggnc atatganaca cnttgatgta ctggccnang
//
