LOCUS       sequence-132701-       10341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-Ago2 S387E mutant lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10341)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pHAGE GFP-Ago2 S387E
FEATURES             Location/Qualifiers
     source          1..10341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3353..3373
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             join(3415..3417,3418..3420,3421..4131)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELXGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3721..3740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4068..4089
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      6064..6073
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6731..7319
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6784..6804)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7202..7213)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7394..7627
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7746..7764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7832..7936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7937..8005,8006..8797)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8155..8174)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8968..9556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9457..9476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9710..9727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9967..9988
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10003..10008,10009..10026,10027..10033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10041..10057
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10046..10068
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10065..10081
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10065..10081
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10131..10150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcatggtg
     3421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct gganggcgac
     3481 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ntacggcaag
     3541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3601 accaccctga cntacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3841 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3901 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3961 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     4021 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     4081 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggatccatg
     4141 tactcgggag ccggccccgc acttgcacct cctgcgccgc cgccccccat ccaaggatat
     4201 gccttcaagc ctccacctag acccgacttt gggacctccg ggagaacaat caaattacag
     4261 gccaatttct tcgaaatgga catccccaaa attgacatct atcattatga attggatatc
     4321 aagccagaga agtgcccgag gagagttaac agggaaatcg tggaacacat ggtccagcac
     4381 tttaaaacac agatctttgg ggatcggaag cccgtgtttg acggcaggaa gaatctatac
     4441 acagccatgc cccttccgat tgggagggac aaggtggagc tggaggtcac gctgccagga
     4501 gaaggcaagg atcgcatctt caaggtgtcc atcaagtggg tgtcctgcgt gagcttgcag
     4561 gcgttacacg atgcactttc agggcggctg cccagcgtcc cttttgagac gatccaggcc
     4621 ctggacgtgg tcatgaggca cttgccatcc atgaggtaca cccccgtggg ccgctccttc
     4681 ttcaccgcgt ccgaaggctg ctctaaccct cttggcgggg gccgagaagt gtggtttggc
     4741 ttccatcagt ccgtccggcc ttctctctgg aaaatgatgc tgaatattga tgtgtcagca
     4801 acagcgtttt acaaggcaca gccagtaatc gagtttgttt gtgaagtttt ggattttaaa
     4861 agtattgaag aacaacaaaa acctctgaca gattcccaaa gggtaaagtt taccaaagaa
     4921 attaaaggtc taaaggtgga gataacgcac tgtgggcaga tgaagaggaa gtaccgtgtc
     4981 tgcaatgtga cccggcggcc cgccagtcac caaacattcc cgctgcagca ggagagcggg
     5041 cagacggtgg agtgcacggt ggcccagtat ttcaaggaca ggcacaagtt ggttctgcgc
     5101 tacccccacc tcccatgttt acaagtcgga caggagcaga aacacaccta ccttcccctg
     5161 gaggtctgta acattgtggc aggacaaaga tgtattaaaa aattaacgga caatcagacc
     5221 tcaaccatga tcagagcgac tgctaggtcg gcgcccgatc ggcaagaaga gattagcaaa
     5281 ttgatgcgaa gtgcagagtt caacacagat ccatacgtcc gtgaatttgg aatcatggtc
     5341 aaagatgaga tgacagacgt gactgggcgg gtgctgcagc cgccctccat cctctacggg
     5401 ggcaggaata aagctattgc gacccctgtc cagggcgtct gggacatgcg gaacaagcag
     5461 ttccacacgg gcatcgagat caaggtgtgg gccattgcgt gcttcgcccc ccagcgccag
     5521 tgcacggaag tccatctgaa gtccttcaca gagcagctca gaaagatctc gagagacgcc
     5581 ggcatgccca tccagggcca gccgtgcttc tgcaaatacg cgcagggggc ggacagcgtg
     5641 gagcccatgt tccggcacct gaagaacacg tatgcgggcc tgcagctggt ggtggtcatc
     5701 ctgcccggca agacgcccgt gtacgccgag gtcaagcgcg tgggagacac ggtgctgggg
     5761 atggccacgc agtgcgtgca gatgaagaac gtgcagagga ccacgccaca gaccctgtcc
     5821 aacctctgcc tgaagatcaa cgtcaagctg ggaggcgtga acaacatcct gctgccccag
     5881 ggcaggccgc cggtgttcca gcagcccgtc atctttctgg gagcagacgt cactcacccc
     5941 cccgccgggg atgggaagaa gccctccatt gccgccgtgg tgggcagcat ggacgcccac
     6001 cccaatcgct actgcgccac cgtgcgcgtg cagcagcacc ggcaggagat catacaagac
     6061 ctggccgcca tggtccgcga gctcctcatc cagttctaca agtccacgcg cttcaagccc
     6121 acccgcatca tcttctaccg cgacggtgtc tctgaaggcc agttccagca ggttctccac
     6181 cacgagttgc tggccatccg tgaggcctgt atcaagctag aaaaagacta ccagcccggg
     6241 atcaccttca tcgtggtgca gaagaggcac cacacccggc tcttctgcac tgacaagaac
     6301 gagcgggttg ggaaaagtgg aaacattcca gcaggcacga ctgtggacac gaaaatcacc
     6361 caccccaccg agttcgactt ctacctgtgt agtcacgctg gcatccaggg gacaagcagg
     6421 ccttcgcact atcacgtcct ctgggacgac aatcgtttct cctctgatga gctgcagatc
     6481 ctaacctacc agctgtgtca cacctacgtg cgctgcacac gctccgtgtc catcccagcg
     6541 ccagcatact acgctcacct ggtggccttc cgggccaggt accacctggt ggataaggaa
     6601 catgacagtg ctgaaggaag ccatacctct gggcagagta acgggcgaga ccaccaagca
     6661 ctggccaagg cggtccaggt tcaccaagac actctgcgca ccatgtactt tgcttaaatc
     6721 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6781 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6841 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6901 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6961 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7021 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7081 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7141 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7201 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7261 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7321 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7381 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7621 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7681 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     7741 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7801 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7861 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7921 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7981 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8041 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8101 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8161 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8221 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8281 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8341 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8401 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8461 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8521 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8581 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8641 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8701 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8761 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8821 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8881 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9721 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9781 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9841 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9901 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     9961 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10021 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10081 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10141 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10201 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10261 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10321 atgtactaca aacttagtag t
//
