LOCUS       sequence-132726-        4626 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, 
            Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY
  TITLE     A protein-protein interaction network for human inherited ataxias 
            and disorders of Purkinje cell degeneration.
  JOURNAL   Cell. 2006 May 19. 125(4):801-14.
  PUBMED    16713569
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            May 19. 125(4):801-14."
COMMENT     Sequence Label: pENTR-AIF
FEATURES             Location/Qualifiers
     source          1..4626
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2386..2485)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2502..2521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2503..2521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2526..2542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2526..2542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2973..3764
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3857..4445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4346..4365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4599..4616
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagttggcat gttccggtgt ggaggcctgg cggcgggtgc tttgaagcag aagctggtgc
      601 ccttggtgcg gaccgtgtgc gtccgaagcc cgaggcagag gaaccggctc ccaggcaact
      661 tgttccagcg atggcatgtt cctctagaac tccagatgac aagacaaatg gctagctctg
      721 gtgcatcagg gggcaaaatc gataattctg tgttagtcct tattgtgggc ttatcaacag
      781 taggagctgg tgcctatgcc tacaagacta tgaaagagga cgaaaaaaga tacaatgaaa
      841 gaatttcagg gttagggctg acaccagaac agaaacagaa aaaggccgcg ttatctgctt
      901 cagaaggaga ggaagttcct caagacaagg cgccaagtca tgttcctttc ctgctaattg
      961 gtggaggcac agctgctttt gctgcagcca gatccatccg ggctcgggat cctggggcca
     1021 gggtactgat tgtatctgaa gatcctgagc tgccgtacat gcgacctcct ctttcaaaag
     1081 aactgtggtt ttcagatgac ccaaatgtca caaagacact gcgattcaaa cagtggaatg
     1141 gaaaagagag aagcatatat ttccagccac cttctttcta tgtctctgct caggacctgc
     1201 ctcatattga gaatggtggt gtggctgtcc tcactgggaa gaaggtagta cagctggatg
     1261 tgagagacaa catggtgaaa cttaatgatg gctctcaaat aacctatgaa aagtgcttga
     1321 ttgcaacagg aggtactcca agaagtctgt ctgccattga tagggctgga gcagaggtga
     1381 agagtagaac aacgcttttc agaaagattg gagactttag aagcttggag aagatttcac
     1441 gggaagtcaa atcaattacg attatcggtg ggggcttcct tggtagcgaa ctggcctgtg
     1501 ctcttggcag aaaggctcga gccttgggca cagaagtgat tcaactcttc cccgagaaag
     1561 gaaatatggg aaagatcctc cccgaatacc tcagcaactg gaccatggaa aaagtcagac
     1621 gagagggggt taaggtgatg cccaatgcta ttgtgcaatc cgttggagtc agcagtggca
     1681 agttacttat caagctgaaa gacggcagga aggtagaaac tgaccacata gtggcagctg
     1741 tgggcctgga gcccaatgtt gagttggcca agactggtgg cctggaaata gactcagatt
     1801 ttggtggctt ccgggtaaat gcagagctac aagcacgctc taacatctgg gtggcaggag
     1861 atgctgcatg cttctacgat ataaagttgg gaaggaggcg ggtagagcac catgatcacg
     1921 ctgttgtgag tggaagattg gctggagaaa atatgactgg agctgctaag ccgtactggc
     1981 atcagtcaat gttctggagt gatttgggcc ccgatgttgg ctatgaagct attggtcttg
     2041 tggacagtag tttgcccaca gttggtgttt ttgcaaaagc aactgcacaa gacaacccca
     2101 aatctgccac agagcagtca ggaactggta tccgatcaga gagtgagaca gagtccgagg
     2161 cctcagaaat tactattcct cccagcaccc cggcagttcc acaggctccc gtccaggggg
     2221 aggactacgg caaaggtgtc atcttctacc tcagggacaa agtggtcgtg gggattgtgc
     2281 tatggaacat ctttaaccga atgccaatag caaggaagat cattaaggac ggtgagcagc
     2341 atgaagatct caatgaagta gccaaactat tcaacattca tgaagaccca actttcttgt
     2401 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2461 tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt
     2521 acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca
     2581 ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct
     2641 cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     2701 gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct
     2761 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     2821 tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     2881 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     2941 agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat
     3001 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     3061 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
     3121 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     3181 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     3241 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     3301 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     3361 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     3421 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     3481 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     3541 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     3601 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     3661 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     3721 tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat
     3781 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     4021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4621 cggaag
//
