LOCUS       sequence-132735-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2Gi2R assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, 
            McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
            Winkler J, Yao J, Hetzer MW, Gage FH
  TITLE     Directly Reprogrammed Human Neurons Retain Aging-Associated 
            Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic 
            Defects.
  JOURNAL   Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001.
  PUBMED    26456686
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001."
COMMENT     Sequence Label: pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             144..164
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             165..869
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(307..325)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     575..594
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
       61 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
      121 gatgataagc ttgccgtacg atgccaaaaa agaaaagaaa agttgtgagc aagggcgagg
      181 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
      241 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
      301 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      361 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      421 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
      481 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      541 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
      601 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
      661 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
      721 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
      781 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      841 ccaccggcgg catggacgag ctgtacaagt ccggactcag atctcgagct cacatcacaa
      901 gttt
//
