LOCUS       sequence-132737-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2Gi2R assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Mertens J, Paquola AC, Ku M, Hatch E, Bohnke L, Ladjevardi S, 
            McGrath S, Campbell B, Lee H, Herdy JR, Goncalves JT, Toda T, Kim Y,
            Winkler J, Yao J, Hetzer MW, Gage FH
  TITLE     Directly Reprogrammed Human Neurons Retain Aging-Associated 
            Transcriptomic Signatures and Reveal Age-Related Nucleocytoplasmic 
            Defects.
  JOURNAL   Cell Stem Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001.
  PUBMED    26456686
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Oct 6. pii: S1934-5909(15)00408-7. doi: 
            10.1016/j.stem.2015.09.001."
COMMENT     Sequence Label: pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(58..86)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     681..701
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    763..787
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             796..822
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 atttcgaccc ggatccgcgg ccgcatggcc agcaaaggag aagaactttt cactggagtt
       61 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      121 gagggtgaag gtgatgctac atacggaaag cttaccctta aatttatttg cactactgga
      181 aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcaatgcttt
      241 tcccgttatc cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      301 tatgtacagg aacgcactat atctttcaaa gatgacggga actacaagac gcgtgctgaa
      361 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaaaggtat tgattttaaa
      421 gaagatggaa acattctcgg acacaaactc gagtacaact ataactcaca caatgtatac
      481 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacatt
      541 gaagatggat ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      601 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgccctttc gaaagatccc
      661 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
      721 ggcatggatg agctctacaa aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa
      781 gcaggcttac tacagctacc gccgctggaa agactgactc tgggaggcgg gatggtgagc
      841 aagggcgagg agctgttcac cggggtggtg cccatcctgg
//
