LOCUS       sequence-132740-        5207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 5207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-FGFR3
FEATURES             Location/Qualifiers
     source          1..5207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2968..3067)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3084..3103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3085..3103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3108..3124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3108..3124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3554..4345
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4438..5026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4927..4946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5180..5197
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagttggcat gggcgcccct gcctgcgccc tcgcgctctg cgtggccgtg gccatcgtgg
      601 ccggcgcctc ctcggagtcc ttggggacgg agcagcgcgt cgtggggcga gcggcagaag
      661 tcccgggccc agagcccggc cagcaggagc agttggtctt cggcagcggg gatgctgtgg
      721 agctgagctg tcccccgccc gggggtggtc ccatggggcc cactgtctgg gtcaaggatg
      781 gcacagggct ggtgccctcg gagcgtgtcc tggtggggcc ccagcggctg caggtgctga
      841 atgcctccca cgaggactcc ggggcctaca gctgccggca gcggctcacg cagcgcgtac
      901 tgtgccactt cagtgtgcgg gtgacagacg ctccatcctc gggagatgac gaagacgggg
      961 aggacgaggc tgaggacaca ggtgtggaca caggggcccc ttactggaca cggcccgagc
     1021 ggatggacaa gaagctgctg gccgtgccgg ccgccaacac cgtccgcttc cgctgcccag
     1081 ccgctggcaa ccccactccc tccatctcct ggctgaagaa cggcagggag ttccgcggcg
     1141 agcaccgcat tggaggcatc aagctgcggc atcagcagtg gagcctggtc atggaaagcg
     1201 tggtgccctc ggaccgcggc aactacacct gcgtcgtgga gaacaagttt ggcagcatcc
     1261 ggcagacgta cacgctggac gtgctggagc gctccccgca ccggcccatc ctgcaggcgg
     1321 ggctgccggc caaccagacg gcggtgctgg gcagcgacgt ggagttccac tgcaaggtgt
     1381 acagtgacgc acagccccac atccagtggc tcaagcacgt ggaggtgaat ggcagcaagg
     1441 tgggcccgga cggcacaccc tacgttaccg tgctcaagac ggcgggcgct aacaccaccg
     1501 acaaggagct agaggttctc tccttgcaca acgtcacctt tgaggacgcc ggggagtaca
     1561 cctgcctggc gggcaattct attgggtttt ctcatcactc tgcgtggctg gtggtgctgc
     1621 cagccgagga ggagctggtg gaggctgacg aggcgggcag tgtgtatgca ggcatcctca
     1681 gctacggggt gggcttcttc ctgttcatcc tggtggtggc ggctgtgacg ctctgccgcc
     1741 tgcgcagccc ccccaagaaa ggcctgggct cccccaccgt gcacaagatc tcccgcttcc
     1801 cgctcaagcg acaggtgtcc ctggagtcca acgcgtccat gagctccaac acaccactgg
     1861 tgcgcatcgc aaggctgtcc tcaggggagg gccccacgct ggccaatgtc tccgagctcg
     1921 agctgcctgc cgaccccaaa tgggagctgt ctcgggcccg gctgaccctg ggcaagcccc
     1981 ttggggaggg ctgcttcggc caggtggtca tggcggaggc catcggcatt gacaaggacc
     2041 gggccgccaa gcctgtcacc gtagccgtga agatgctgaa agacgatgcc actgacaagg
     2101 acctgtcgga cctggtgtct gagatggaga tgatgaagat gatcgggaaa cacaaaaaca
     2161 tcatcaacct gctgggcgcc tgcacgcagg gcgggcccct gtacgtgctg gtggagtacg
     2221 cggccaaggg taacctgcgg gagtttctgc gggcgcggcg gcccccgggc ctggactact
     2281 ccttcgacac ctgcaagccg cccgaggagc agctcacctt caaggacctg gtgtcctgtg
     2341 cctaccaggt ggcccggggc atggagtact tggcctccca gaagtgcatc cacagggacc
     2401 tggctgcccg caatgtgctg gtgaccgagg acaacgtgat gaagatcgca gacttcgggc
     2461 tggcccggga cgtgcacaac ctcgactact acaagaagac aaccaacggc cggctgcccg
     2521 tgaagtggat ggcgcctgag gccttgtttg accgagtcta cactcaccag agtgacgtct
     2581 ggtcctttgg ggtcctgctc tgggagatct tcacgctggg gggctccccg taccccggca
     2641 tccctgtgga ggagctcttc aagctgctga aggagggcca ccgcatggac aagcccgcca
     2701 actgcacaca cgacctgtac atgatcatgc gggagtgctg gcatgccgcg ccctcccaga
     2761 ggcccacctt caagcagctg gtggaggacc tggaccgtgt ccttaccgtg acgtccaccg
     2821 acgagtacct ggacctgtcg gcgcctttcg agcagtactc cccgggtggc caggacaccc
     2881 ccagctccag ctcctcaggg gacgactccg tgtttgccca cgacctgctg cccccggccc
     2941 cacccagcag tgggggctcg cggacgttgc caactttctt gtacaaagtt ggcattataa
     3001 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     3061 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     3121 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     3181 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     3241 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     3301 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3361 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3421 gtttttttgg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     3481 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     3541 aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     3601 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     3661 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     3721 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     3781 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     3841 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     3901 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     3961 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     4021 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     4081 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     4141 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     4201 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     4261 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     4321 gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat cccttaacgt
     4381 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4561 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5101 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     5161 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
