LOCUS       sequence-132756-        6232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Kendall SD, Linardic CM, Adam SJ, Counter CM
  TITLE     A network of genetic events sufficient to convert normal human cells
            to a tumorigenic state.
  JOURNAL   Cancer Res. 2005 Nov 1. 65(21):9824-8.
  PUBMED    16267004
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2005 Nov 1. 65(21):9824-8."
COMMENT     Sequence Label: pbabe-cyclinD1+CDK4R24C
FEATURES             Location/Qualifiers
     source          1..6232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             120..589
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     regulatory      1665..1674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1681..2010)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1695..1830
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1995..2015
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      2306..2315
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2913..2922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2957..3373)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(2973..2989)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(3005..3027)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    3574..3773
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             3838..4307
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        4338..4442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4443..4511,4512..5303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4661..4680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5463..6051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5952..5971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6205..6222
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
       61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
      121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
      241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
      301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
      421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
      481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
      541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
      601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      721 ttctggagac taaataaaat cttttatttt atcgattcac tccggattac cttcatcctt
      781 atgtagataa gagtgctgca gagctcgaaa ggcagagatt cgcttgtgtg ggttaaaagt
      841 cagcatttcc agcagcagct gtgctcccga ctcctccatc tcaggtacca ccgactgcac
      901 tgggcggggc cctctggggg gaaaggctcc acggggcagg gatacatctc gaggccagtc
      961 atcctctgga ggcagcccaa tcaggtcaaa gattttgccc aactggtcgg cttcagagtt
     1021 tccacagaag agaggctttc gacgaaacat ctctgcaaag atacagccaa cactccacat
     1081 gtccacaggt gttgcatatg tggactgcag aagaacttcg ggagctcggt accagagtgt
     1141 aacaaccacg ggtgtaagtg ccatctggta gctgtagatt ctggccaggc caaagtcagc
     1201 cagcttgact gttccaccac ttgtcaccag aatgttctct ggcttcagat ctcggtgaac
     1261 gatgcaattg gcatgaagga aatctaggcc tcttagaaac tggcgcatca gatccttgat
     1321 cgtttcggct ggcaagcctg gtgggggtgc cttgtccaga tatgtcctta ggtcctggtc
     1381 tacatgctca aacaccaggg ttaccttgat ctcccggtca gttcgggatg tggcacagac
     1441 gtccatcagc cggacaacat tgggatgctc aaaagcctcc agtcgcctca gtaaagccac
     1501 ctcacgaact gtgctgatgg gaaggcctcc tccacctcct cctccattgg ggactctcac
     1561 actcttgagg gccacaaagt ggccactgtg gggatcacag gccttgtaca ctgtcccata
     1621 ggcaccgaca ccaatttcag ccactggctc atatcgagag gtagccatgg tggcaagctt
     1681 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
     1741 cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg
     1801 ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc
     1861 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     1921 acacctggtt gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc
     1981 tggggacttt ccacacccta actgacacac attccacagg gtcgaccctc agatgtccac
     2041 gtcccgcacg tcggtgggtg tgcaagccag gtccacctcc tcctcctcct cttcctcctc
     2101 ctcggcggcc ttggggtcca tgttctgctg ggcctggcgc aggcttgact ccagcagagc
     2161 ttcgatctgc tcctggcagg cccggaggca gtctgggtca cacttgatca ctctggagag
     2221 gaagcgtgtg aggcggtagt aggacaggaa gttgttgggg ctcctcaggt tcaggccttg
     2281 cactgcggcc accacgctcc ccgctgccac catggagggc ggattggaaa tgaacttcac
     2341 atctgtggca cagagggcaa cgaaggtctg cgcgtgtttg cggatgatct gtttgttctc
     2401 ctccgcctct ggcattttgg agaggaagtg ttcaatgaaa tcgtgcgggg tcattgcggc
     2461 caggttccac ttgagcttgt tcaccaggag cagctccatt tgcagcagct cctcgggccg
     2521 gatggagttg tcggtgtaga tgcacagctt ctcggccgtc agggggatgg tctccttcat
     2581 cttagaggcc acgaacatgc aagtggcccc cagcagctgc aggcggctct ttttcacggg
     2641 ctccagcgac aggaagcggt ccaggtagtt catggccagc gggaagacct cctcctcgca
     2701 cttctgttcc tcgcagacct ccagcatcca ggtggcgacg atcttccgca tggacggcag
     2761 gacctccttc tgcacacatt tgaagtagga caccgagggc gcgcaggtct cctccgcctt
     2821 cagcatggcc cgcagcaccc ggtcgttgag gaggttggca tcggggtacg cgcggcggat
     2881 ggtttccact tcgcagcaca ggagctggtg ttccatggtg gcgaattcct acgtaccacc
     2941 acactgggat ccggccggcg cctagagaag gagtgagggc tggataaagg gaggatcgag
     3001 gcggggtcga acgaggaggt tcaaggggga gagacggggc ggatggagga agaggaggcg
     3061 gaggcttagg gtgtacaaag ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc
     3121 aggtcacgat gtaggggacc tggtctgggt gtccatgcgg gccaggtgaa aagaccttga
     3181 tcttaacctg ggtgatgagg tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa
     3241 aggttggcca ttctgcagag cagaaggtaa cccaacgtct cttcttgaca tctaccgact
     3301 ggttgtgagc gatccgctcg acatctttcc agtgacctaa ggtcaaactt aagggagtgg
     3361 taacagtctg gccctaattt tcagacaaat acagaaacac agtcagacag agacaacaca
     3421 gaacgatgct gcagcgctgc agcagacaag acgcgcggct tcggttccaa accgaaagca
     3481 aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga accacatatc
     3541 ctgacggggt cggattccac atcgactccc ttcctcaggt cgggccacaa aaacggcccc
     3601 caaagtccct gggacgtctc ccagggttgc ggccgggtgt tcagaactcg tcagttccac
     3661 cacgggtccg ccagatacag agctagttag ctaactagta ccgacgcagg cgcataaaat
     3721 cagtcataga cactagacaa tcggacagac acagataagt tgctggccag cttgcctccc
     3781 ggtggtgggt cggtggtccc tgggcagggg tctcccgatc ccggacgagc ccccaaatga
     3841 aagacccccg ctgacgggta gtcaatcact cagaggagac cctcccaagg aacagcgaga
     3901 ccacaagtcg gatgcaactg caagagggtt tattggatac acgggtaccc gggcgactca
     3961 gtcaatcgga ggactggcgc cccgagtgag gggttgtggg ctcttttatt gagctcgggg
     4021 agcagaagcg cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca
     4081 gggtcatttc aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg
     4141 agggctggac cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct
     4201 taccacagat atcctgtttg gcccatattc agctgttcca tctgttcttg gccctgagcc
     4261 ggggcaggaa ctgcttacca cagatatcct gtttggccca tattcaggct gcaggtggca
     4321 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4441 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4501 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4561 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4621 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4681 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4741 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4801 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4861 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4921 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4981 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5041 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5101 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5161 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5221 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5281 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5341 atttgcggcc gcaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5401 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5581 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5821 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
