LOCUS       sequence-132764-       12322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Bagnyukova TV, Restifo D, Beeharry N, Gabitova L, Li T, Serebriiskii
            IG, Golemis EA, Astsaturov I
  TITLE     DUSP6 regulates drug sensitivity by modulating DNA damage response.
  JOURNAL   Br J Cancer. 2013 Aug 20;109(4):1063-71. doi: 10.1038/bjc.2013.353. 
            Epub 2013 Jul 9.
  PUBMED    23839489
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/bjc.2013.353"; journalName: "Br J Cancer"; date: 
            "2013-08-20- 20"; volume: "109"; issue: "4"; pages: "1063-71"
COMMENT     Sequence Label: DUSP6
FEATURES             Location/Qualifiers
     source          1..12322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(971..2173)
                     /codon_start=1
                     /gene="tetA from transposon Tn10"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFGV
                     LLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRLLS
                     GITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIMGGFAGEISPHSPFFI
                     AALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYFSAQL
                     IGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRVATKWGEKTAVLLG
                     FIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGALQGLLVS
                     LTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQAQGSKQET
                     SA"
     protein_bind    2182..2200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    2314..2448
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             2616..3249
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3296..3421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3918..4151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4336..4380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4678..4795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4860..5234)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      5231..5240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5244..5262)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5254..5301)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     enhancer        5406..5709
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5710..5913
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5864..5884
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5910..5934
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6032..6058
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6038..6064
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    6077..6101
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7248..7272)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7292..7321
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    7368..7940
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7535..7552)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7762..7781
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7943..8545
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     8442..8462
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8552..9140
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8605..8625)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9023..9034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9347..9580
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9681..9698)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9687..9911
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9957..10385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10044..10063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10254..10275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10394..10414)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10399..10728
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10579..10714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10641..10660
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             10777..11802
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             10777..11802
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    11932..12053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12102..12118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12102..12118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12115..12137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12126..12142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12150..12156,12157..12174,12175..12180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12195..12216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgacaga gtcacgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
      901 gaatcgggag cggcgatacc ctatgcatca agcttggtac cgagctcgga tcgttaatta
      961 attaacgatc cgcacttgtc tcctgtttac tcccctgagc ttgaggggtt aacatgaagg
     1021 tcatcgatag caggataata atacagtaaa acgctaaacc aataatccaa atccagccat
     1081 cccaaattgg tagtgaatga ttgtaaataa cagcaaacag taatgggcca ataacaccgg
     1141 ttgcattggt aaggctcacc aataatccct gtaaagcacc ttgctgatga ctctttgttt
     1201 ggatagacat cactccctgt aatgcaggta acgcgatccc accaccagcc aataaaatta
     1261 aaacagggaa aactaaccaa ccttcagata taaacgctaa aaaggcaaat gcactactat
     1321 ctgcaataaa tccgagcagt actgccgttt tttcgcccca tttagtggct actcttcctg
     1381 ccacaaaggc ttggaatact gagtgtaaaa gaccaagacc cgctaatgaa aagccaacca
     1441 tcatgctatt ccatccaaaa cgattttcgg taaatagcac ccacaccgtt gcgggaattt
     1501 ggcctatcaa ttgcgctgaa aaataaataa tcaacaaaat gggcatcgtt ttaaataaag
     1561 tgatgtatac cgaattcgat tgcgtctcaa cccctacttc ggtatctgta ttatcacgtg
     1621 tatttttggt ttcacggaac caaaacataa ccacaaggaa agtgacaata tttagcaacg
     1681 cagcgataaa aaagggacta tgcggtgaaa tctctcctgc aaaaccaccc ataataggcc
     1741 ccgctattaa accaagccca aaacttgccc ctaaccaacc gaaccacttc acgcgttgag
     1801 aggctgaggt ggtatcggca atgaccgatg ccgcgacagc cccagtagct cctgtgatcc
     1861 ctgaaagcaa acggcctaaa tacagcatcc aaagcgcact tgaaaaagcc agcaataagt
     1921 aatccagcga tgcgcctatt aatgacaaca acagcaccgg gcgccgacca aatcggtcag
     1981 acatttttcc aagccaagga gcaaagataa cctgcattaa cgcataaagt gcaagcaata
     2041 cgccaaagtg gttagcgatg tcttccgaag caataaattc acgtaataac gttggcaaga
     2101 ctggcatgat aaggccaatc cccatggcat cgagtaacgt aattaccaat gcgatctttg
     2161 tcgaactatt catttcactt ttctctatca ctgataggga gtattgtctc atgagcggat
     2221 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2281 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     2341 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     2401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     2461 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     2521 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     2581 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     2641 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     2701 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     2761 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     2821 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     2881 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     2941 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     3001 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     3061 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3241 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     3781 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     3841 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     3901 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     3961 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     4021 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     4081 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     4141 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     4201 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     4261 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     4321 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     4381 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     4441 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     4501 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     4561 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     4621 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     4681 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     4741 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     4801 ttattacagg gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat
     4861 cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac
     4921 tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag
     4981 ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc
     5041 caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg
     5101 tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg
     5161 tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg
     5221 gtcaacttgg ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta
     5281 tgccgatgat taattgtcaa cacgtgctgc aggtccgagg ttctagacgt attaccgcca
     5341 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5401 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     5461 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     5521 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     5581 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     5641 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     5701 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     5761 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5821 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     5881 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     5941 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
     6001 actctagcct aggccgcgga ccatgggatc ctatccgtat gatgttcctg attatgctag
     6061 cctcgggtac cggatcacaa gtttgtacaa aaaagcaggc ttcatgatag atacgctcag
     6121 acccgtgccc ttcgcgtcgg aaatggcgat cagcaagacg gtggcgtggc tcaacgagca
     6181 gctggagctg ggcaacgagc ggctgctgct gatggactgc cggccgcagg agctatacga
     6241 gtcgtcgcac atcgagtcgg ccatcaacgt ggccatcccg ggcatcatgc tgcggcgcct
     6301 gcagaagggt aacctgccgg tgcgcgcgct cttcacgcgc ggcgaggacc gggaccgctt
     6361 cacccggcgc tgtggcaccg acacagtggt gctctacgac gagagcagca gcgactggaa
     6421 cgagaatacg ggcggcgagt cggtgctcgg gctgctgctc aagaagctca aggacgaggg
     6481 ctgccgggcg ttctacctgg aaggtggctt cagtaagttc caagccgagt tctccctgca
     6541 ttgcgagacc aatctagacg gctcgtgtag cagcagctcg ccgccgttgc cagtgctggg
     6601 gctcgggggc ctgcggatca gctctgactc ttcctcggac atcgagtctg accttgaccg
     6661 agaccccaat agtgcaacag actcggatgg tagtccgctg tccaacagcc agccttcctt
     6721 cccagtggag atcttgccct tcctctactt gggctgtgcc aaagactcca ccaacttgga
     6781 cgtgttggag gaattcggca tcaagtacat cttgaacgtc acccccaatt tgccgaatct
     6841 ctttgagaac gcaggagagt ttaaatacaa gcaaatcccc atctcggatc actggagcca
     6901 aaacctgtcc cagtttttcc ctgaggccat ttctttcata gatgaagccc ggggcaagaa
     6961 ctgtggtgtc ttggtacatt gcttggctgg cattagccgc tcagtcactg tgactgtggc
     7021 ttaccttatg cagaagctca atctgtcgat gaacgatgcc tatgacattg tcaaaatgaa
     7081 aaaatccaac atatccccta acttcaactt catgggtcag ctgctggact tcgagaggac
     7141 gctgggactc agcagcccat gtgacaacag ggttccagca cagcagctgt attttaccac
     7201 cccttccaac cagaatgtat accaggtgga ctctctgcaa tctacggacc cagctttctt
     7261 gtacaaagtg gttcgatcta gaatggctag cgaacaaaag ctcatatctg aagaggacct
     7321 atagaattct cgagtctaga gggcccgacg cgtctggcca ccggtcgccc ctctccctcc
     7381 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     7441 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     7501 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     7561 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     7621 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     7681 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     7741 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     7801 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     7861 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     7921 ctttgaaaaa cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac
     7981 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     8041 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     8101 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8161 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8221 ctcgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8281 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8341 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8401 gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8461 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8521 catgacccgc aagcccggtg cctgagttaa caatcaacct ctggattaca aaatttgtga
     8581 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     8641 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     8701 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     8761 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     8821 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     8881 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     8941 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     9001 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     9061 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     9121 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     9181 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     9241 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     9301 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     9361 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9421 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9481 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9541 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9601 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     9661 tgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     9721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     9781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     9841 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     9901 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     9961 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    10021 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    10081 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    10141 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    10201 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    10261 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    10321 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    10381 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    10441 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    10501 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    10561 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    10621 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    10681 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    10741 gcttgtatat ccattttcgg atctgatcag cacgtgatga aaaagcctga actcaccgcg
    10801 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
    10861 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
    10921 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
    10981 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
    11041 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
    11101 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
    11161 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
    11221 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
    11281 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
    11341 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
    11401 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
    11461 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
    11521 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
    11581 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
    11641 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
    11701 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
    11761 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agcacgtgct acgagatttc
    11821 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
    11881 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
    11941 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    12001 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    12061 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
    12121 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12181 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12241 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12301 ggcggtttgc gtattgggcg ct
//
