LOCUS       sequence-132770-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD
  TITLE     Two-stage dynamic deregulation of metabolism improves process 
  JOURNAL   Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. 
            Epub 2021 Sep 30.
  PUBMED    34600151
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-11"; volume: "68:106-118. doi"; pages: " 
            10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30"
COMMENT     Sequence Label: pCASCADE-gltA1-gltA2
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(412..439)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(455..526)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(611..630)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 ggcggggttt ttttttcggt ttatccccgc tggcgcgggg aactcgaact aataactgtc
       61 ccgaatgaat tggtcaatac ggtttatccc cgctggcgcg gggaactcga acttcataac
      121 ttttacgcat tatatgcttt tcggtttatc cccgctggcg cggggaactc gaggtggtac
      181 cagatctttt gaattcaaag ttaaacaaaa ttatttgtag agggaaaccg ttgtggtctc
      241 cctgaatata cgcgttttat gctcgcgggg taacatgaca tgctcgaatt acagaaaaat
      301 aacttttttg ttacatttgt aagatagtaa ggtgtcagaa agagttatcc agcaaccact
      361 catagaccta gggcagcaga tagggacgac gtggtgttag ctgtgtataa acgcagaaag
      421 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
      481 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggatgatg
      541 tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
      601 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
      661 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
      721 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
      781 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
      841 ggtacacggc ctacagaaa
//
