LOCUS       sequence-132771-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA1-gltA2 targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD
  TITLE     Two-stage dynamic deregulation of metabolism improves process 
  JOURNAL   Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. 
            Epub 2021 Sep 30.
  PUBMED    34600151
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-11"; volume: "68:106-118. doi"; pages: " 
            10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30"
COMMENT     Sequence Label: pCASCADE-gltA1-gltA2
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(444..461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taacaccacg tcgtccctat ctgctgccct aggtctatga gtggttgctg gataactctt
       61 tctgacacct tactatctta caaatgtaac aaaaaagtta tttttctgta attcgagcat
      121 gtcatgttac cccgcgagca taaaacgcgt atattcaggg agaccacaac ggtttccctc
      181 tacaaataat tttgtttaac tttgaattca aaagatctgg taccacctcg agttccccgc
      241 gccagcgggg ataaaccgaa aagcatataa tgcgtaaaag ttatgaagtt cgagttcccc
      301 gcgccagcgg ggataaaccg tattgaccaa ttcattcggg acagttatta gttcgagttc
      361 cccgcgccag cggggataaa ccgaaaaaaa aaccccgccc ctgacagggc ggggtttttt
      421 ttcctaggga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
      481 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
      541 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
      601 acaagcatca cgaaa
//
