LOCUS       sequence-132778-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. 
            coli Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Ye Z, Li S, Hennigan JN, Lebeau J, Moreb EA, Wolf J, Lynch MD
  TITLE     Two-stage dynamic deregulation of metabolism improves process 
  JOURNAL   Metab Eng. 2021 Nov;68:106-118. doi: 10.1016/j.ymben.2021.09.009. 
            Epub 2021 Sep 30.
  PUBMED    34600151
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2021-11"; volume: "68:106-118. doi"; pages: " 
            10.1016/j.ymben.2021.09.009. Epub 2021 Sep 30"
COMMENT     Sequence Label: pSMART-Ala2
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(15..44)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     terminator      128..199
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      215..242
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag ttgattgcag
       61 tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa ttcgtttgcc
      121 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      181 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      241 cgtttataca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt
      301 gctggataac gtgcgtaatt gtgctgatct cttatatagc tgctctcatt atctctctac
      361 cctgaagtga ctctctcacc tgtaaaaata atatctcaca ggcttaatag tttcttaata
      421 caaagcctgt aaaacgtcag gataacttct atattcaggg agaccacaac ggtttccctc
      481 tacaaataat tttgtttaac tttcgtagaa gagcacttca cacttctgga aaaaggagat
      541 ataccatgat cattggggtg ccgaaggaga tcaaaaataa tgagaaccgc gtcgcgttga
      601 ccccgggagg tgtcagccag ctgatctcta atggccatcg tgtcttagtt gaaacaggcg
      661 ctggcctggg ttctggcttc gaaaacgagg cctacgaatc tgcaggtgcg gaaattattg
      721 ctgatccaaa acaggtctgg gatgcagaga tggtcatgaa agtgaaagaa ccgctcccgg
      781 aagaatatgt ctattttcgt aaaggtctgg tgctgtttac atatctgcat ctggcagctg
      841 aaccggagct cgcacaagcc cttaaagata a
//
