LOCUS       sequence-132787-        8133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mEos2 photoconvertible fluorescent protein fused to the N-terminus 
            of isoform 1 of Cep123 (Cep89/CCDC123).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Sillibourne JE, Specht CG, Izeddin I, Hurbain I, Tran P, Triller A, 
            Darzacq X, Dahan M, Bornens M
  TITLE     Assessing the localization of centrosomal proteins by PALM/STORM 
            nanoscopy.
  JOURNAL   Cytoskeleton (Hoboken). 2011 Nov;68(11):619-27. doi: 
            10.1002/cm.20536. Epub 2011 Oct 18.
  PUBMED    21976302
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.20536"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2011-11"; volume: 
            "68"; issue: "11"; pages: "619-27"
COMMENT     Sequence Label: pLP-mEos2-Cep123
FEATURES             Location/Qualifiers
     source          1..8133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..678
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     protein_bind    complement(718..751)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(3223..3317)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3338..3997)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3912..3931
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(4026..4059)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4316..4437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4353..4372)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4407..4426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4444..4899)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4581..4602)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4793..4812
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4926..5030
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5032..5389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5032..5223
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5055..5075)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5240..5375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5424..6218
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5424..6218
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5478..5497)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6088..6107
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6406..6425)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6450..6497
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6826..7414
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7315..7334
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7589..7892
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7893..8096
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8047..8067
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg
       61 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
      121 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
      181 ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag
      241 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
      301 tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
      361 tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
      421 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct
      481 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
      541 gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc
      601 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
      661 cctgacaatg ccagacgact gtacaagtcc ggactcagat ctcgagctca agcttcgata
      721 acttcgtata gcatacatta tacgaagtta tcagtcgaca tgctcctggg atttcggaga
      781 ggccgcagga gtcatttcaa acacatcatc catggccttt tacctgcagc cagcgttgct
      841 ccgaaggcag ctgtgccacg cacacctcct ccccgcagcc ccaacccatc tccagagaga
      901 ccaagatctg ctctggcagc agccattctg gcgacaacat tgactgggcg gacggttgct
      961 attcctcagc ctcgccagag gtcccggtct gagagtgatg tgagcagtgt tgaacaggac
     1021 agcttcatcg agccctatgc caccacctca cagctgaggc ctcggccaaa ttggcagagt
     1081 gagatgggaa gaagatcttc tttgccatcc tttgaaacac tggactatgg ggacgaagag
     1141 gacattgaaa ctcagctgtc atccagcggc aaggaattgg gggatgtcag tgcccgggag
     1201 gacagaggag gccacagtga tgacctgtac gctgtgccac acagaaatca ggtgccattg
     1261 ttacatgagg tgaacagtga agacgatgaa aatatttctc atcaagatgg gtttccaggc
     1321 tcccctcctg caccacagcg gacacaacaa aaagatggta aacaccctgt tctgaattta
     1381 aaggatgaaa aacctccatt atgtgagaaa cctccaccct ccccagatat aactggtaga
     1441 gcacgtcaaa gatatacaga aataaccaga gaaaagtttg aggcattaaa agaagaaaat
     1501 atggacctaa acaatatgaa tcaaagcctt acccttgaac taaacacaat gaaacaagca
     1561 atgaaagaac tacagttaaa acttaaggga atggaaaaag agaagagaaa gctcaaagag
     1621 gctgagaagg cgtcgtcaca ggaagttgct gcacctgaat tactttatct gcgaaaacaa
     1681 gctcaagaac tggtggatga aaatgatgga ttgaaaatga ctgtccatcg tttgaatgta
     1741 gaactcagtc gatatcagac aaaattcagg catttgtcca aggaagagag cttaaatatt
     1801 gaaggcctcc catccaaggg ccctatacca ccctggttgt tggatataaa gtacctgtca
     1861 ccattgttgc tggcttatga agatatgatg aaagagaagg acgagctcaa tgccaccctc
     1921 aaggaggaaa tgagaatgtt taggatgcga gtccaagaag tggtgaaaga aaatgaagaa
     1981 ttgcaccaag agttaaataa gagtagtgct gttaccagtg aggaatggcg tcagcttcag
     2041 actcaagcaa aactggtttt agaggaaaac aagttgttgc tggagcagtt ggagattcag
     2101 caaaggaaag ccaaggacag ccaccaggag cgcctccaag aagtttctaa gctgactaaa
     2161 caactaatgc tcctggaggc aaaaacccac ggccaggaaa aggagctggc ggagaacagg
     2221 gaacagctgg agattttacg tgccaaatgc caagaactca aaacacactc ggatggcaaa
     2281 atcgcagtgg aagttcataa atcaattgtg aatgaattaa aaagccaatt acagaaggaa
     2341 gaagagaaag aaagggctga gatggaggag ttgatggaga agctgacagt cctgcaagcg
     2401 cagaagaaga gcctgctgtt agagaagaac agtttgacag agcaaaacaa agcactggaa
     2461 gccgaacttg aacgagcaca gaaaatcaat aggaaatctc aaaagaaaat tgaggtcctc
     2521 aaaaagcagg tggaaaaagc catggggaac gaaatgtctg ctcatcagta cctggcaaac
     2581 cttgttggcc tggcagaaaa tataacccag gaacgtgaca gtcttatgtg tttggcaaaa
     2641 tgtttagaaa gtgagaagga tggagtgctt aataaagtca taaaaagcaa cattcgcctg
     2701 ggaaagttag aggaaaaagt caagggctac aagaagcagg cagcactgaa gctgggggac
     2761 atcagtcacc gtctgctgga gcagcaggag gacttcgccg gcaagacagc ccagtaccgg
     2821 caggagatgc ggcacctgca ccaggtgctg aaggacaagc aggaggtgct ggaccaggcg
     2881 ctgcagcaga acagagaaat ggaaggtgaa cttgaagtta tttgggaatc taccttcagg
     2941 gaaaaccgaa gaatccgaga acttctccag gacacactca cgaggacagg cgtgcaggac
     3001 aaccccagag ctctggttgc ccccagcctc aatggcgtct ctcaggcaga cctgctggac
     3061 ggctgcgatg tctgctccta tgacctgaag tctcatgccc ccacctgcta gggatccgct
     3121 cgagaagctt tctagaccat tcgtttggcg cgcgggccca gtaggtaagt gaacatggtc
     3181 atagctgttt cctaggagat cctggtcatg actagtgctt ggattctcac caataaaaaa
     3241 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
     3301 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
     3361 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
     3421 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
     3481 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
     3541 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     3601 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     3661 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     3721 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     3781 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     3841 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     3901 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     3961 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     4021 gatccataac ttcgtatagc atacattata cgaagttata gatccaatat tattgaagca
     4081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtggat ccaccggatc
     4201 tagataactg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     4261 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     4321 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg
     4441 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     4501 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     4561 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     4621 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     4681 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4741 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4801 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4861 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     4921 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4981 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     5041 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5101 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5161 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     5221 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5281 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5341 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     5401 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5461 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5521 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5581 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     5641 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5701 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5761 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     5821 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     5881 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     5941 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6001 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6061 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6121 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6181 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6241 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6301 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6361 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     6421 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     6481 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     6541 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     6601 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     6661 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6961 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7141 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7201 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7261 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7321 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7381 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7441 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7501 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     7561 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7621 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7681 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     7741 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7801 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7861 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     7921 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     7981 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     8041 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     8101 tagtgaaccg tcagatccgc tagcgctacc ggt
//
