LOCUS       sequence-132789-         405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of SnoopCatcher, an engineered protein forming 
            a spontaneous covalent bond with its cognate peptide partner 
            SnoopTag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Veggiani G, Nakamura T, Brenner MD, Gayet RV, Yan J, Robinson CV, 
            Howarth M
  TITLE     Programmable polyproteams built using twin peptide superglues.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201519214.
  PUBMED    26787909
REFERENCE   2  (bases 1 to 405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2016 Jan 19. pii: 201519214."
COMMENT     Sequence Label: pET28a-SnoopCatcher
FEATURES             Location/Qualifiers
     source          1..405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             64..399
                     /label=SnoopCatcher
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgaagccgc tgcgtggtgc cgtgtttagc ctgcagaaac agcatcccga ctatcccgat
      121 atctatggcg cgattgatca gaatgggacc tatcaaaatg tgcgtaccgg cgaagatggt
      181 aaactgacct ttaagaatct gagcgatggc aaatatcgcc tgtttgaaaa tagcgaaccc
      241 gctggctata aaccggtgca gaataagccg attgtggcgt ttcagattgt gaatggcgaa
      301 gtgcgtgatg tgaccagcat tgtgccgcag gatattccgg ctacatatga atttaccaac
      361 ggtaaacatt atatcaccaa tgaaccgata ccgccgaaat aataa
//
