LOCUS       sequence-132797-        9406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP16
FEATURES             Location/Qualifiers
     source          1..9406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(783..1382)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(866..886)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1363..1382
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1403..1902)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1451..1470)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1844..1867
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    1921..1945
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4412..4436)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4452..4478)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4491..4514)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4514..4523
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4596..4620)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4630..4648
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4651..4669
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4671..4874)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4700..4720)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4875..5254
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5269..5685)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5285..5301)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5317..5339)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5752..6093
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             6157..6673
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6873..6892)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7020..7042)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7236..7255)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7355..7377
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7415..7433)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7501..7605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7606..7674,7675..8466)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7824..7843)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8637..9225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9126..9145
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9379..9396
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa
      241 ctatttcagg agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac
      301 ttaatattcg actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat
      361 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      421 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgacg
      481 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      541 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      601 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagcttgcca
      661 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
      721 tttatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
      781 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
      841 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
      901 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
      961 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     1021 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     1081 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     1141 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     1201 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     1261 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     1321 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     1381 atggtaagct tgggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg
     1441 cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg
     1501 ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag
     1561 cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc
     1621 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     1681 acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag
     1741 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg
     1801 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
     1861 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgttatcga
     1921 accactttgt acaagaaagt tgggtacagt attctctcat aaaataggag gtacgcttgt
     1981 gagtttagta ctttagttgt aggcacagct tgcacatgtg tgtcgctgat gtgaaaccac
     2041 tgcccttttg attccatttc aaaatcttgt ggaatatcac cgtgaagaac aagattagag
     2101 agatgactat ttgcggttct tgccttggca taggcagtgt aatgccccga cctcatagta
     2161 ccactgtgtt caacaactcc atataaggaa tagagtaccc ttgtattttc ttctgcaaca
     2221 ttcttacatt taagggtgca aaaaggagcc aaatctaaga tttccggaaa ctttatgtgt
     2281 ttgttaactt tgcgtaggtt aaaaccagcc tgctgaaatc tctttaaatg aagagtaaga
     2341 acaggaggag caagagaaat tagcatctgc tttttggcat tggtgtaaac atgcttcctt
     2401 tcacctttta tatttgcctt tggtccatta cactgtctcc gtgtgcatac ttcacaaagc
     2461 agtttattcg catctcgaag tttctcatta cgggtgaact gatataaaca atgttggatt
     2521 gaacactcat cagtattgaa aacttccctg tttgcaagag tacagaaagc agtttctgga
     2581 tcttcattta caacctcata cacctttgtt ccaggagtat gactatcatt cagaatctct
     2641 atatttattt catcaggatg aagagcagca ttcaaattta ggtttttgaa accattggaa
     2701 atgtccactt ctccattgct cccttccgtt aggtaggcac catttaaatt cctagtggga
     2761 gaagatgtta aaacctccag atcattatcc atgttgatat ttttcatatc tacttcctct
     2821 gtggattttt gattgtcagt tacactttcg atcatttttg cttggccatt caaatctttc
     2881 tggttgacac aatattcttt atgcataaca ccctcttgtg aaatatggtt ggatttaata
     2941 tttacttctc cttgaagtga catttcagct tcattatcac tgtcttcagg atggtcaata
     3001 gtacaaatat catttaaatg aagaactttt ccttgaattt tttgttgtct tcgttggttc
     3061 ttggcttgct ttttggcttg tttctttgct tttttctgta agtgcttact tgttccagaa
     3121 ggaatatcac ttctctcttt tatgtaactg tcgttatctt tttcttcctc actatcttga
     3181 tcttcatcct ccactgtctt tttcagattt ttatcattta cacttttctt accactctga
     3241 tcatctaaaa ctgggaggga caaatcaagg aaagattcat gaaccaagga gacagttctg
     3301 cattgatcac acatgatcat actagttagt tcaccaccaa agatgcggtc aacaaaactt
     3361 ggcattgatt ttttcttctc ataatcttta actttatttt ttagttcttc atccaacttt
     3421 tcagtagaat taccaaatgc tttaagtatt cctttactca ctctttggtg ttcttctgct
     3481 ctcatcccat ccaataagta gcgaagcagc tcctggctgt cttgctgctg atagccttta
     3541 aaccgcactg cttttttaca gacctgagaa aagagttctt tcggtgtcac aacccccttt
     3601 ttggtctctt gcatctcatt aagaaactgg ctcatggcta aagtaagagg gcctggaggc
     3661 tcaaggttta tttctaatgg ttctgttaat gccaaatcag gtggttcaat ttttacaatt
     3721 gttccagaca ttttcacttc ttttagtagt tctctaagca ctggtgtttg tgacaagttc
     3781 tgcataactg cattgaagaa acatgtgttt cccaaattac tgagtccttt cacggttatt
     3841 tggcaaggag aattcatggg aggattctct ttagccatgt tttccttctt ttctctctct
     3901 tgttcattct tactctcttt ttctaatttt ttattttcaa gttcaatatt tccattatct
     3961 ttctctggct ttggagttgt aatgctggca tgttttctga cataatcaac cacttgaccc
     4021 aactggtttg aactacaata ctggacctca ttatcacata cgtaacacca tacactccag
     4081 ttgtccaaac taagaaccag acagtgaggt tcagatcttg gcgtcagata gtgcttcaag
     4141 gcatgctgct cctgagaatt tctgccacag ccctgatggc cacatttaag acacagccaa
     4201 actgaaggct tttcttctgt ttcttcttca gctttatctt tcactttatt gtcagtctta
     4261 cagtcttggc agatattcca ttccacattc actaaagcct ttttcaaatt accttgttcc
     4321 aatccttttc taatgtgtct gcacacaggt tctaaagttt cagaggaatc atcgattgga
     4381 acagtttttc ccttagtccg tttctttccc atgccaactt ttttgtacaa acttgtttga
     4441 taacctcgag cagcgtaatc tggaacatcg tatgggtatc ctccatcgag cttgtcatcg
     4501 tcatccttgt agtccatggt ggcagatccc cggaggctgg atcggtcccg gtgtcttcta
     4561 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
     4621 cgtcgacgat ctctatcact gatagggaga tctctatcac tgatagggag agctctgctt
     4681 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     4741 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     4801 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     4861 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     4921 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     4981 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     5041 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     5101 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     5161 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     5221 gattactatt aataactagt caataatcaa tgtcaaggat ctaattccgg cgcctagaga
     5281 aggagtgagg gctggataaa gggaggatcg aggcggggtc gaacgaggag gttcaagggg
     5341 gagagacggg gcggatggag gaagaggagg cggaggctta gggtgtacaa agggcttgac
     5401 ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg atgtagggga cctggtctgg
     5461 gtgtccatgc gggccaggtg aaaagacctt gatcttaacc tgggtgatga ggtctcggtt
     5521 aaaggtgccg tctcgcggcc atccgacgtt aaaggttggc cattctgcag agcagaaggt
     5581 aacccaacgt ctcttcttga catctaccga ctggttgtga gcgatccgct cgacatcttt
     5641 ccagtgacct aaggtcaaac ttaagggagt ggtaacagtc tggccctaat tttcagacaa
     5701 atacagaaac acagtcagac agagacaaca cagaacgatg ctgcagcgct gcagcagaca
     5761 agacgcgcgg cttcggttcc aaaccgaaag caaaaattca gacggaggcg ggaactgttt
     5821 taggttctcg tctcctacca gaaccacata tcctgacggg gtcggattcc acatcgactc
     5881 ccttcctcag gtcgggccac aaaaacggcc cccaaagtcc ctgggacgtc tcccagggtt
     5941 gcggccgggt gttcagaact cgtcagttcc accacgggtc cgccagatac agagctagtt
     6001 agctaactag tacagacgca ggcgcaaaca ttagatgccg gcacaaacac gcacaaagac
     6061 agagacagac acgaaacgac cgctggccag cttacctccc ggcggggggg tcggtggtcc
     6121 ctgggcaggg gtctccaaat ctcggtggaa cctccaaatg aaagaccccc gaggtgggca
     6181 gtcaatcaat ctgaggagac cctcccaagg atcagcgagt ccacgattcg gatgcaaaca
     6241 gcaagaggct ttattgggaa tacgggtacc cgggcgacgc agtctatcgg aggactggcg
     6301 cgccgagtga ggggttgtgg gctcttttat tgagctcggg gagcagaagc gcgcgaacag
     6361 aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt caggtccttg
     6421 gggcaccctg gaaacatctg atggttctct agaaactgct gagggcggga ccgcatctgg
     6481 ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga tatcctgttt
     6541 ggcccatatt ctgctgtctc tctgttccta accttgatct gaacttctct attctcagtt
     6601 atgtattttc catgccttgc aaaatggcgt tacttaagct agcttgccaa acctacaggt
     6661 ggggtctttc attcccccct ttttctggag actaaataaa atcttttatt ttatctatgg
     6721 ctcgtactct ataggcttca gctggtgata ttgttgagtc aaaactagag cctggaccac
     6781 tgatatcctg tctttaacaa attggactaa tcgcctctac gccggacgca tcgtggccgg
     6841 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga
     6901 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg
     6961 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcgccgtc
     7021 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7081 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7141 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     7201 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7261 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7321 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7381 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     7441 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     7501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     7621 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     7681 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     7741 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     7801 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     7861 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     7921 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     7981 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     8041 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     8101 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     8161 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     8221 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     8281 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     8341 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     8401 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     8461 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     8521 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     8581 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     8641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     8701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     8761 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     8821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     8881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     8941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9001 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9181 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9241 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     9301 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     9361 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaag
//
