LOCUS       sequence-132798-        9295 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP1
FEATURES             Location/Qualifiers
     source          1..9295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(783..1382)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(866..886)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     1363..1382
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(1403..1902)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1451..1470)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1844..1867
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    1921..1945
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4301..4325)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4341..4367)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(4380..4403)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      4403..4412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4485..4509)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4519..4537
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4540..4558
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(4560..4763)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4589..4609)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4764..5143
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5158..5574)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5174..5190)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5206..5228)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    5641..5982
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             6046..6562
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6762..6781)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6909..6931)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7125..7144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7244..7266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7304..7322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7390..7494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7495..7563,7564..8355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7713..7732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8526..9114
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9015..9034
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9268..9285
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacaattctc cccagctctt tctgcgctgt ccataatgaa
      241 ctatttcagg agtattggag tatgtggatt caaaacagtg gctaattgag tcaggattac
      301 ttaatattcg actcctaccc tcagaatgtt gttctccaac ttcaagaaac tgttacccat
      361 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
      421 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgacg
      481 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
      541 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
      601 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagcttgcca
      661 aacctacagg tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat
      721 tttatcgatg catggggtcg tgcgctcctt tcggtcgggc gctgcgggtc gtggggcggg
      781 cgtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
      841 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
      901 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
      961 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     1021 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     1081 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     1141 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     1201 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     1261 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     1321 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     1381 atggtaagct tgggctgcag gtcgaaaggc ccggagatga ggaagaggag aacagcgcgg
     1441 cagacgtgcg cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg
     1501 ccccgcccct gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag
     1561 cccagaaagc gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc
     1621 attgctcagc ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc
     1681 acgtcctgca cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag
     1741 aaggtggcgc gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg
     1801 aatgtgtgcg aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag
     1861 cgcatgctcc agactgcctt gggaaaagcg cctcccctac ccggtagaat tcgttatcga
     1921 accactttgt acaagaaagt tgggtataat ttcttataaa atagcaagta aggagtagaa
     1981 gtaggagatg tagaagggga aagagaattc agaaagtcct tctcttcagt aactttgact
     2041 tcagaatcat caaaaagcaa ccacttcccc tcatactcct ttaagctttg cactacgtat
     2101 gaaattttgt tctcaaatcc actaatatta atgcctgttt ggtcactgga ttccttgttt
     2161 ctatcagatt catgggtccc agtagtatca ctagtctcag aatttctatt ttcagcaaac
     2221 gctgtactag caaccttatc aggattagag gctttgttgt atagctcata atctgctttg
     2281 ctcttttgtc ctccaagaag tccaatagct tctacatttt ttttgttcaa aactttactt
     2341 ggctgtgtat ttccaccaac tctaattgac acttcttcat cattataatt ctcaaccaca
     2401 ccccttgctt cctcctcatt caatggttct ggcttaccta tttcacacat ttggtcaacc
     2461 acaaaatttc ctttatctag ttctaaactg ttaaggtcag tgactttaac agaagcagtg
     2521 taatgcccac tactaattgt aatgccacta tgcatcacaa ccgcaaataa tccatagctg
     2581 tcgttagttg gctttgtgct ccattcttct agtgacaatt taagaggtgt caataaagga
     2641 gtgttgatct tggaaagtcc accaccataa caatcaaact ccaaaccact agcagcaaag
     2701 cacttcaaat gaatagttat aacttcaggc attttgtcaa acaaaagact tcgttcagct
     2761 tcagtataat gatggcagtt ttcacagaaa tatttatctt ctcctacaat cctttctact
     2821 gaagcaaatt gtgaaattgc ccatctcagg gtcttcattt ctgtttttgg ctctggagaa
     2881 atttcagaac tctcctctac tttggaaagc tcatcttctt gtactggcac actgatgtct
     2941 tgaaaatctt ctcttctttc tgttaaactt tcacattcca agcaacgcgt ccttaatacc
     3001 agctgacctt gaaataattt ctccactagc tcaaaaccaa tttgttcttc acctttgttt
     3061 atgggtttaa cttcattaac atttacaggt gtaacagtat ttcctggaga ttcaagtcca
     3121 caaccattag ttgtgttatc actttcacac ttccccaagt cctcttcagg atcacattca
     3181 ttttctttag attgtccttt gactccttgg tttgttgtta tttttcccag actacaaaat
     3241 ttagaaagaa tgctgggttg cttagttgca gactttaacc aatttatttt aactcttgat
     3301 ttcttttgtg agggtcttgg actctcattt tcagaaatat acttgggaat tattttagga
     3361 ggactctcta atgtatcact tgtagctttt ctttttgatc tagtttgtct ctggttctct
     3421 tccaatgact ggtgttcctt ggataattta acttttttct tcatgttacc aaattcagtg
     3481 tcactttttc ttttcccatt tccttttggg agtttctctt taaagtcttc agaatgcctc
     3541 atactgtcca tctctatgct gttaatacca ttcatttcct ctttcggatg aggtatttct
     3601 tctaccttag taggtaattc tgccacattt tttacttctt ctttttttag gagttggcat
     3661 gtttcttgaa tgtttcccaa aatacattgt aatacttcct gtgcatcatg ctgtagatat
     3721 ccttcataca tagggttgag ttccctcagt gtgttaagca gtcgccttgg ctgagtggca
     3781 agttcatcag tatatttctc tggatttaag agaaaactag cctggagctg ttcaaccgaa
     3841 atgattaagg actgtaaact gcatatcaat tcataacttg ccaaagaatc ttctttgcaa
     3901 tttcccttgt ctttttgatt ggcttcatcc tttagagctt ctttcttcct tgaaataata
     3961 ttaaataagt gctttactcc agatttaaaa ccgggacaaa aatataatac ctgaagtata
     4021 ctattaagat agcaagtatt gccgagatta ttcagtccca caaatggtaa caagttttct
     4081 ctcttctcac agtttatagg tgaagactgt gctgcaggaa caacttgatc aatttcagat
     4141 gctctatatt cagaagcttt ttcttcattt tcttgagaat ctgtgaaatc caaagctctc
     4201 ttagtttcct ttttctgaaa aaactttaag gaaagtctgt ttttctttga agggctacct
     4261 cttgaaagtc cattactttc actaggtatg acaccaggca tgccaacttt tttgtacaaa
     4321 cttgtttgat aacctcgagc agcgtaatct ggaacatcgt atgggtatcc tccatcgagc
     4381 ttgtcatcgt catccttgta gtccatggtg gcagatcccc ggaggctgga tcggtcccgg
     4441 tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg acggttcact
     4501 aaacgagctc gtcgacgatc tctatcactg atagggagat ctctatcact gatagggaga
     4561 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     4621 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     4681 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     4741 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     4801 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     4861 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     4921 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     4981 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     5041 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     5101 cccgtaattg attactatta ataactagtc aataatcaat gtcaaggatc taattccggc
     5161 gcctagagaa ggagtgaggg ctggataaag ggaggatcga ggcggggtcg aacgaggagg
     5221 ttcaaggggg agagacgggg cggatggagg aagaggaggc ggaggcttag ggtgtacaaa
     5281 gggcttgacc cagggagggg ggtcaaaagc caaggcttcc caggtcacga tgtaggggac
     5341 ctggtctggg tgtccatgcg ggccaggtga aaagaccttg atcttaacct gggtgatgag
     5401 gtctcggtta aaggtgccgt ctcgcggcca tccgacgtta aaggttggcc attctgcaga
     5461 gcagaaggta acccaacgtc tcttcttgac atctaccgac tggttgtgag cgatccgctc
     5521 gacatctttc cagtgaccta aggtcaaact taagggagtg gtaacagtct ggccctaatt
     5581 ttcagacaaa tacagaaaca cagtcagaca gagacaacac agaacgatgc tgcagcgctg
     5641 cagcagacaa gacgcgcggc ttcggttcca aaccgaaagc aaaaattcag acggaggcgg
     5701 gaactgtttt aggttctcgt ctcctaccag aaccacatat cctgacgggg tcggattcca
     5761 catcgactcc cttcctcagg tcgggccaca aaaacggccc ccaaagtccc tgggacgtct
     5821 cccagggttg cggccgggtg ttcagaactc gtcagttcca ccacgggtcc gccagataca
     5881 gagctagtta gctaactagt acagacgcag gcgcaaacat tagatgccgg cacaaacacg
     5941 cacaaagaca gagacagaca cgaaacgacc gctggccagc ttacctcccg gcgggggggt
     6001 cggtggtccc tgggcagggg tctccaaatc tcggtggaac ctccaaatga aagacccccg
     6061 aggtgggcag tcaatcaatc tgaggagacc ctcccaagga tcagcgagtc cacgattcgg
     6121 atgcaaacag caagaggctt tattgggaat acgggtaccc gggcgacgca gtctatcgga
     6181 ggactggcgc gccgagtgag gggttgtggg ctcttttatt gagctcgggg agcagaagcg
     6241 cgcgaacaga agcgagaagc gaactgattg gttagttcaa ataaggcaca gggtcatttc
     6301 aggtccttgg ggcaccctgg aaacatctga tggttctcta gaaactgctg agggcgggac
     6361 cgcatctggg gaccatctgt tcttggccct gagccggggc aggaactgct taccacagat
     6421 atcctgtttg gcccatattc tgctgtctct ctgttcctaa ccttgatctg aacttctcta
     6481 ttctcagtta tgtattttcc atgccttgca aaatggcgtt acttaagcta gcttgccaaa
     6541 cctacaggtg gggtctttca ttcccccctt tttctggaga ctaaataaaa tcttttattt
     6601 tatctatggc tcgtactcta taggcttcag ctggtgatat tgttgagtca aaactagagc
     6661 ctggaccact gatatcctgt ctttaacaaa ttggactaat cgcctctacg ccggacgcat
     6721 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     6781 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     6841 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     6901 tgcgccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6961 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7021 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     7081 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     7141 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7201 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     7261 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     7321 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7381 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7441 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7501 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7561 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7621 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7681 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7741 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7801 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7861 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7921 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7981 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8041 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8101 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8161 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8221 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8281 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8341 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8401 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8461 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8521 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8581 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8641 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8701 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8761 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8821 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8881 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8941 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9001 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9061 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9121 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9181 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9241 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
