LOCUS       sequence-132801-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C 
            Virus (HCV) infection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Ren Q, Li C, Yuan P, Cai C, Zhang L, Luo GG, Wei W
  TITLE     A Dual-Reporter System for Real-Time Monitoring and High-throughput 
            CRISPR/Cas9 Library Screening of the Hepatitis C Virus.
  JOURNAL   Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865.
  PUBMED    25746010
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-03-9"; volume: "5:8865. doi"; pages: " 
            10.1038/srep08865"
COMMENT     Sequence Label: pLenti-NIrD
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(23..70)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     293..309
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     294..310
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(544..654)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
ORIGIN
        1 tggcatggtt agttctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
       61 aattgtcaac acgtgctcga gatgtctgtt tctggggttg tgagtcaccc gccggatgtc
      121 agaaacccgc cggatgtcag aaacccgccg gatgtcagaa acccgccgga tgtcagaaac
      181 ccgccggatg tcagaagaat tcacccgccg gatgtcagaa acccgccgga tgtcagaaac
      241 ccgccggatg tcagaaaccc gccggatgtc agaaacccgc cggactagtg attcgaggtc
      301 gacggtatcg atctagtgca gacgccgccg gtacagcacc accaggagtg tgactaccag
      361 cacccctgtc acagccacct ggagccacag agccccaggt gagggcctgt ggcatggcac
      421 ctccctctcc tggaacttcc ggtcggcttg tggcctgggg ccgccatccg ggtccgcagg
      481 tggcccaggg ttgccctcca ggagctggat gctggggttc tcagccacgt ggatcccccc
      541 ggggagcatg tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa
      601 gtcgtcaagg gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggtcgg
      661 cccgccgctt tcgcacttta gctgtttctc caggccacat atgattagtt ccaggccgaa
      721 aaggaaggca ggttcggctc cctgccggtc gaacagctca attgcttgtc tcagaagtgg
      781 gggcatagaa tcggtggtag gtgtctctct ttcctctttt gctacttgat gctcctgttc
      841 ctccaatacg cagcccagtg taaagtggcc cacggcggac agagcgtaca gtgcgttctc
      901 cagggagaag ccttgctgac acaggaacgc gagctgattt tccagggttt cgtactgttt
      961 ctctgttggg cggggtgccg agatgcactt ttagccccgt cgcgatgtga ga
//
