LOCUS       sequence-132805-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 5 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1delltaH5
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             637..654
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(707..725)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      721..768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(892..911)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcatgaa aacctgtatt ttcagggcag cacctttagc aaactgcgtg
      121 aacagctggg cccggtgacc caggaatttt gggataacct ggaaaaagaa accgaaggcc
      181 tgcgtcagga aatgagcaaa gatctggaag aggtgaaagc gaaagtgcag ccgtatctgg
      241 atgactttca gaaaaaatgg caggaagaga tggaactgta tcgtcagaaa gtggaaccgc
      301 tgggcgaaga gatgcgtgat cgtgcgcgtg cgcatgtgga tgcgctgcgt acccatctgg
      361 cgccgtatag cgatgaactg cgtcagcgtc tggcggcccg tctggaagcg ctgaaagaaa
      421 acggcggtgc gcgtctggcg gaatatcatg cgaaagcgac cgaacatctg agcaccctga
      481 gcgaaaaagc gaaaccggcg ctggaagatc tgcgtcaggg cctgctgccg gtgctggaaa
      541 gctttaaagt gagctttctg agcgcgctgg aagagtatac caaaaaactg aacacccagt
      601 aagagctccg tcgacaagct tgcggccgca ctcgagcacc accaccacca ccactgagat
      661 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      721 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      781 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      841 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      901 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      961 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     1021 agggtgatgg ttc
//
