LOCUS       sequence-132806-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 5 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1delltaH5
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(589..609)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(610..627)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             646..668
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    683..707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(707..726)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(708..726)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(810..829)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcttactg ggtgttcagt tttttggtat actcttccag
      121 cgcgctcaga aagctcactt taaagctttc cagcaccggc agcaggccct gacgcagatc
      181 ttccagcgcc ggtttcgctt tttcgctcag ggtgctcaga tgttcggtcg ctttcgcatg
      241 atattccgcc agacgcgcac cgccgttttc tttcagcgct tccagacggg ccgccagacg
      301 ctgacgcagt tcatcgctat acggcgccag atgggtacgc agcgcatcca catgcgcacg
      361 cgcacgatca cgcatctctt cgcccagcgg ttccactttc tgacgataca gttccatctc
      421 ttcctgccat tttttctgaa agtcatccag atacggctgc actttcgctt tcacctcttc
      481 cagatctttg ctcatttcct gacgcaggcc ttcggtttct ttttccaggt tatcccaaaa
      541 ttcctgggtc accgggccca gctgttcacg cagtttgcta aaggtgctgc cctgaaaata
      601 caggttttca tgatgatgat gatgatggct gctgcccatg gtatatctcc ttcttaaagt
      661 taaacaaaat tatttctaga ggggaattgt tatccgctca caattcccct atagtgagtc
      721 gtattaattt cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat
      781 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      841 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      901 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
      961 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     1021 gcgtcgagat cccg
//
