LOCUS       sequence-132807-        9679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Kimura E, Han JJ, Li S, Fall B, Ra J, Haraguchi M, Tapscott SJ, 
            Chamberlain JS
  TITLE     Cell-lineage regulated myogenesis for dystrophin replacement: a 
            novel therapeutic approach for treatment of muscular dystrophy.
  JOURNAL   Hum Mol Genet. 2008 Aug 15. 17(16):2507-17.
  PUBMED    18511457
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2008 Aug 15. 17(16):2507-17."
COMMENT     Sequence Label: pLv-CMV-MyoD
FEATURES             Location/Qualifiers
     source          1..9679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        288..514
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             515..695
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    742..867
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1778..1822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2084..2201
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2293..2596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2597..2800
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2751..2771
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2797..2821
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          2949..3327
                     /label=chimeric intron
                     /note="fusion between human cytomegalovirus intron A and 
                     human beta-globin intron 2"
     LTR             6485..6718
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6790..6911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6827..6846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6881..6900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6951..7086
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7106..7125)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7107..7125)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7135..7152)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7135..7151)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7144..7166)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7293..7748
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7380..7399)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7590..7611
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7774..7878
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7879..7947,7948..8739)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8097..8116)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8910..9498
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9399..9418
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9652..9669
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     2041 agacagatcc attcgattag tgaacggatc tcgacggtat cggttttaaa agaaaagggg
     2101 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2161 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcggggct gcaggaattg
     2221 gccgcacgcg tctagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2581 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2641 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     2701 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     2761 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     2821 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     2881 cctccgcgga ttcgaatccc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
     2941 agagtgacgt aagtaccgcc tatagagtct ataggcccac aaaaaatgct ttcttctttt
     3001 aatatacttt tttgtttatc ttatttctaa tactttccct aatctctttc tttcagggca
     3061 ataatgatac aatgtatcat gcctctttgc accattctaa agaataacag tgataatttc
     3121 tgggttaagg caatagcaat atttctgcat ataaatattt ctgcatataa attgtaactg
     3181 atgtaagagg tttcatattg ctaatagcag ctacaatcca gctaccattc tgcttttatt
     3241 ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg
     3301 ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc
     3361 catcactttg gcaaagaatt gggattcgaa catcgattga attcggacac ctctgacagg
     3421 acaggacagg gaggaggggt agaggacagc cggtgtgcat tccaacccac agaacctttg
     3481 tcattgtact gttggggttc cggagtggca gaaagttaag acgactctca cggcttgggt
     3541 tgaggctgga cccaggaact gggatatgga gcttctatcg ccgccactcc gggacataga
     3601 cttgacaggc cccgacggct ctctctgctc ctttgagaca gcagacgact tctatgatga
     3661 tccgtgtttc gactcaccag acctgcgctt ttttgaggac ctggacccgc gcctggtgca
     3721 cgtgggagcc ctcctgaaac cggaggagca cgcacacttc tctactgcgg tgcacccagg
     3781 cccaggcgct cgtgaggatg agcatgtgcg cgcgcccagc gggcaccacc aggcgggtcg
     3841 ctgcttgctg tgggcctgca aggcgtgcaa gcgcaagacc accaacgctg atcgccgcaa
     3901 ggccgccacc atgcgcgagc gccgccgcct gagcaaagtg aatgaggcct tcgagacgct
     3961 caagcgctgc acgtccagca acccgaacca gcggctaccc aaggtggaga tcctgcgcaa
     4021 cgccatccgc tacatcgaag gtctgcaggc tctgctgcgc gaccaggacg ccgcgccccc
     4081 tggcgccgct gccttctacg cacctggacc gctgccccca ggccgtggca gcgagcacta
     4141 cagtggcgac tcagacgcgt ccagcccgcg ctccaactgc tctgatggca tgatggatta
     4201 cagcggcccc ccaagcggcc cccggcggca gaatggctac gacaccgcct actacagtga
     4261 ggcggtgcgc gagtccaggc cagggaagag tgcggctgtg tcgagcctcg actgcctgtc
     4321 cagcatagtg gagcgcatct ccacagacag ccccgctgcg cctgcgctgc ttttggcaga
     4381 tgcaccacca gagtcgcctc cgggtccgcc agagggggca tccctaagcg acacagaaca
     4441 gggaacccag accccgtctc ccgacgccgc ccctcagtgt cctgcaggct caaaccccaa
     4501 tgcgatttat caggtgcttt gagagatcga ctgcagcagc agagggcgca ccaccgtagg
     4561 cactcctggg gatggtgccc ctggttcttc acgcccaaaa gatgaagctt aaatgacact
     4621 cttcccaact gtcctttcga agccgttctt ccagagggaa gggaagagca gaagtctgtc
     4681 ctagatccag ccccaaagaa aggacatagt cctttttgtt gttgttgttg tagtccttca
     4741 gttgtttgtt tgttttttca tgcggctcac agcgaaggcc acttgcactc tggctgcacc
     4801 tcactgggcc agagctgatc cttgagtggc caggcgctct tcctttcctc atagcacagg
     4861 ggtgagcctt gcacacctaa gccctgccct ccacatcctt ttgtttgtca ctttctggag
     4921 ccctcctggc acccactttt ccccacagct tgcggaggcc actcaggtct caggtgtaac
     4981 aggtgtaacc ataccccact ctcccccttc ccgcggttca ggaccactta tttttttata
     5041 taagactttt gtaatctatt cgtgtaaata agagttgctt ggccagagcg ggagcccctt
     5101 gggctatatt tatctcccag gcatgctgtg tagtgcaaca aaaactttgt atgtttattc
     5161 ctcaagcggg cgagtcaggt gttggaaatc cgaattcccc ggggatcaat tcggcgcgcc
     5221 acgcgtccgc ggactagtct cgagttaatt aagctagcct agtgccattt gttcagtggt
     5281 tcgtagggct ttcccccact gtttggcttt cagttatatg gatgatgtgg tattgggggc
     5341 caagtctgta cagcatcttg agtccctttt taccgctgtt accaattttc ttttgtcttt
     5401 gggtatacat ttaaacccta acaaaacaaa gagatggggt tactctctaa attttatggg
     5461 ttatgtcatt ggatgttatg ggtccttgcc acaagaacac atcatacaaa aaatcaaaga
     5521 atgttttaga aaacttccta ttaacaggcc tattgattgg aaagtatgtc aacgaattgt
     5581 gggtcttttg ggttttgctg ccccttttac acaatgtggt tatcctgcgt tgatgccttt
     5641 gtatgcatgt attcaatcta agcaggcttt cactttctcg ccaacttaca aggcctttct
     5701 gtgtaaacaa tacctgaacc tttaccccgt tgcccggcaa cggccaggtc tgtgccaagt
     5761 gtttgctgac gcaaccccca ctggctgggg cttggtcatg ggccatcagc gcatgcgtgg
     5821 aaccttttcg gctcctctgc cgatccatac tgcggaactc ctagccgctt gttttgctcg
     5881 cagcaggtct ggagcaaaca ttatcgggac tgataactct gttgtcctat cccgcaaata
     5941 tacatcgttt ccatggctgc taggctgtgc tgccaactgg atcctgcgcg ggacgtcctt
     6001 tgtttacgtc ccgtcggcgc tgaatcctgc ggacgaccct tctcggggtc gcttgggact
     6061 ctctcgtccc cttctccgtc tgccgttccg accgaccacg gggcgcacct ctctttacgc
     6121 ggactccccg tctgtgcctt ctcatctgcc ggaccgtgtg cacttcgctt cacctctgca
     6181 cgtcgcatgg agaccaccgt gaacgcccac caaatattgc ccaaggtctt acataagagg
     6241 actcttggac tctcagcaat gtcaacgacc gaccttgagg catacttcaa agactgtttg
     6301 tttaaagact gggaggagtt gggggaggag attaggttaa aggtctttgt actaggaggc
     6361 tgtaggcata aattggtctg cgcaccagca ccatggcgca atcactagag cggggtacct
     6421 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6481 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     6541 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6601 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6661 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     6721 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     6781 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     6841 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6901 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6961 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7021 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7081 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     7141 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     7201 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     7261 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     7321 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     7381 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     7441 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     7501 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     7561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     7621 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     7681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     7741 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7801 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7861 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7921 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7981 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     8041 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     8101 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     8161 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     8221 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     8281 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     8341 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     8401 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     8461 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8521 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8581 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8641 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8701 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8761 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8821 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8881 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8941 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     9001 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     9061 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     9121 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     9181 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     9241 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     9301 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     9361 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     9421 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9481 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9541 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9601 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9661 gtgagcgagg aagcggaag
//
