LOCUS       sequence-132812-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1deltaH4/2H5
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..95
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             601..618
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(671..689)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      685..732
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(856..875)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcatgaaaac ctgtattttc agggcagcac ctttagcaaa ctgcgtgaac
      121 agctgggccc ggtgacccag gaattttggg ataacctgga aaaagaaacc gaaggcctgc
      181 gtcaggaaat gagcaaagat ctggaagagg tgaaagcgaa agtgcagccg tatctggatg
      241 actttcagaa aaaatggcag ccgctgggcg aagagatgcg tgatcgtgcg cgtgcgcatg
      301 tggatgcgct gcgtacccat ctggcgccgt atagcgatga actgcgtcag cgtctggcgg
      361 cccgtctgga agcgctgaaa gaaaacggcg gtgcgcgtct ggcggaatat catgcgaaag
      421 cgaccgaaca tctgagcacc ctgagcgaaa aagcgaaacc ggcgctggaa gatctgcgtc
      481 agggcctgct gccggtgctg gaaagcttta aagtgagctt tctgagcgcg ctggaagagt
      541 ataccaaaaa actgaacacc cagtaagagc tccgtcgaca agcttgcggc cgcactcgag
      601 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
      661 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      721 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
      781 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
      841 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
      901 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
      961 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     1021 agacgg
//
