LOCUS       sequence-132813-        1042 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 1042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1deltaH4/2H5
FEATURES             Location/Qualifiers
     source          1..1042
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(558..578)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(579..596)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             615..637
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    652..676
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(676..695)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(677..695)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(779..798)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcttac tgggtgttca gttttttggt atactcttcc
      121 agcgcgctca gaaagctcac tttaaagctt tccagcaccg gcagcaggcc ctgacgcaga
      181 tcttccagcg ccggtttcgc tttttcgctc agggtgctca gatgttcggt cgctttcgca
      241 tgatattccg ccagacgcgc accgccgttt tctttcagcg cttccagacg ggccgccaga
      301 cgctgacgca gttcatcgct atacggcgcc agatgggtac gcagcgcatc cacatgcgca
      361 cgcgcacgat cacgcatctc ttcgcccagc ggctgccatt ttttctgaaa gtcatccaga
      421 tacggctgca ctttcgcttt cacctcttcc agatctttgc tcatttcctg acgcaggcct
      481 tcggtttctt tttccaggtt atcccaaaat tcctgggtca ccgggcccag ctgttcacgc
      541 agtttgctaa aggtgctgcc ctgaaaatac aggttttcat gatgatgatg atgatggctg
      601 ctgcccatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
      661 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc
      721 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      781 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      841 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      901 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      961 ttcctaatgc aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg
     1021 caaaaccttt cgcggtatgg ca
//
