LOCUS       sequence-132827-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q 
            mutant version) fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: ML1Nmut_in_pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    643..724
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(680..699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 agtccggact cagatcttat tctgatctga ctatggctac acctgccggg agacgcgcaa
       61 gcgaaactga aagactgctg acaccaaacc ctggctatgg aacccaagtg ggcacatctc
      121 cagctcccac cacacccact gaggaagagg acctgcagca gcagctgaag tacttcttta
      181 tgagtccatg cgatcagttc caggcaaagg ggcagcagcc ctgtaaactg atgctgagaa
      241 tcctgacaat ggccactccc gctggcagga gagcaagcga gactgaaagg ctgctgaccc
      301 ctaacccagg ctacggaacc caggtcggaa catccccagc acctactacc cctaccgaag
      361 aggaagacct gcaacaacag ctgaaatatt tctttatgag cccttgcgat cagtttcagg
      421 ccaaggggca gcagccatgt aaactgatgc tggacccccc tgacctggat aattaaggat
      481 ccgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
      541 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      601 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      721 ctgcattcta gttgtggttt gtccaa
//
