LOCUS       sequence-132828-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 
            wildtype version) fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: ML1Nwt_in_pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    643..764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(680..699)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     734..753
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agtccggact cagatcttac tctgacctga ctatggcaac acctgctgga cggagggcaa
       61 gcgagaccga acggctgctg acacccaacc ccggatacgg cactcaagtg ggcacatctc
      121 cagctcccac cacacccact gaggaagagg acctgaggag acggctgaag tacttcttta
      181 tgagtccatg cgataaattc cgcgcaaagg ggcgaaaacc ctgtaagctg atgctgagaa
      241 tcctgacaat ggcaactcca gctggacgac gagcaagcga gactgaaaga ctgctgaccc
      301 ctaacccagg ctacggaacc caggtcggaa catccccagc acctactacc cctaccgaag
      361 aggaagacct gagaaggcgg ctgaagtatt tctttatgag cccttgcgat aaatttcggg
      421 ccaaagggcg caagccatgt aaactgatgc tggacccccc tgacctggat aattaaggat
      481 ccgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
      541 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      601 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
      661 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
      721 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
      781 cgttaatatt ttgttaaa
//
