LOCUS       sequence-132834-         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving dog Caveolin1-minSOG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: Cav1-miniSOG
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..441)
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     regulatory      438..447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
      121 ttatccatcc agctgcactc cgatgaaata ctggagctca cccttctggt ccctcatggg
      181 ctggaggtgc aggaggttcc agaatttctt cccgcttttt gtgtagttta tcagctgcac
      241 ggtaatctcg cgctgatctc taatggcatc gcgaatcttc tgcactgtgg cctggtctgt
      301 ctctggtccc tgcagaaagc ggccattgcg gcccaggatc tcctctctgg aatactcggt
      361 cagctccagg aagccatcgg atgcgaagat gatgggattg tctggcagcc gtggatcagt
      421 tatcacgaaa cttttctcca tggtggcgac cggtggatcc cgtgtttctt tctgcatgtt
      481 gatgcggata ttgctgaata ttttgccaac agcctcaaag aacgggtcac agaaggtgtg
      541 gacgtagatg gaatagacac ggctgatgca ctgaatctca atcaggaaac tcttaatgca
      601 cggcacaact gcccagatgt gcaggaaaga aagaatggca aagtaaatgc cccatatgag
      661 tgccattggg atgccaaaga gggcagacag caagcggtaa aaccagtatt ttgtcacagt
      721 gaaggtggtg aagctggcct tccagatgcc atcaa
//
