LOCUS       sequence-132835-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q 
            mutant version) fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: ML1Nmut_in_pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(682..703)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accgtcgact gcagaattcg gatccttaat tatccaggtc
      181 aggggggtcc agcatcagtt tacatggctg ctgccccttg gcctgaaact gatcgcaagg
      241 gctcataaag aaatatttca gctgttgttg caggtcttcc tcttcggtag gggtagtagg
      301 tgctggggat gttccgacct gggttccgta gcctgggtta ggggtcagca gcctttcagt
      361 ctcgcttgct ctcctgccag cgggagtggc cattgtcagg attctcagca tcagtttaca
      421 gggctgctgc ccctttgcct ggaactgatc gcatggactc ataaagaagt acttcagctg
      481 ctgctgcagg tcctcttcct cagtgggtgt ggtgggagct ggagatgtgc ccacttgggt
      541 tccatagcca gggtttggtg tcagcagtct ttcagtttcg cttgcgcgtc tcccggcagg
      601 tgtagccata gtcagatcag aataagatct gagtccggac ttgtacagct cgtccatgcc
      661 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      721 ggggtctttg ctcagg
//
