LOCUS       sequence-132836-         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 
            wildtype version) fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: ML1Nwt_in_pEGFP-C1
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(682..703)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
       61 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcagtta tctagatccg
      121 gtggatcccg ggcccgcggt accgtcgact gcagaattcg gatccttaat tatccaggtc
      181 aggggggtcc agcatcagtt tacatggctt gcgccctttg gcccgaaatt tatcgcaagg
      241 gctcataaag aaatacttca gccgccttct caggtcttcc tcttcggtag gggtagtagg
      301 tgctggggat gttccgacct gggttccgta gcctgggtta ggggtcagca gtctttcagt
      361 ctcgcttgct cgtcgtccag ctggagttgc cattgtcagg attctcagca tcagcttaca
      421 gggttttcgc ccctttgcgc ggaatttatc gcatggactc ataaagaagt acttcagccg
      481 tctcctcagg tcctcttcct cagtgggtgt ggtgggagct ggagatgtgc ccacttgagt
      541 gccgtatccg gggttgggtg tcagcagccg ttcggtctcg cttgccctcc gtccagcagg
      601 tgttgccata gtcaggtcag agtaagatct gagtccggac ttgtacagct cgtccatgcc
      661 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      721 ggggtctttg ctcagggcgg actgg
//
