LOCUS       sequence-132842-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of Flag-tagged human Dcp2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Wang Z, Jiao X, Carr-Schmid A, Kiledjian M
  TITLE     The hDcp2 protein is a mammalian mRNA decapping enzyme.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):12663-8. Epub 2002 Sep 
            6.
  PUBMED    12218187
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-10-1"; volume: "99(20)"; pages: 
            "12663-8. Epub 2002 Sep 6"
COMMENT     Sequence Label: pCDNA3-Flag-hDcp2
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctgccacc atggactaca aggacgacga tgacaagaag
      121 cttggatcca tcatggagac caaacgggtg gagattcccg gcagcgtcct ggacgatctc
      181 tgcagccgat ttattttgca tattcccagc gaggaaagag acaatgcaat ccgagtgtgt
      241 tttcagattg aacttgccca ttggttttac ttggatttct acatgcagaa cacaccagga
      301 ttacctcagt gtgggataag agactttgct aaagctgtct tcagtcattg tccgtttttg
      361 ctgcctcaag gtgaagatgt ggaaaaagtt ttggatgaat ggaaggaata taaaatggga
      421 gtaccaacat atggtgcaat tattcttgat gagacacttg aaaatgtact actggttcag
      481 gggtacctag caaaatcagg ctggggattt ccaaaaggaa aagtaaataa agaagaagct
      541 cctcatgatt gtgctgctag agaggtcttt gaagaaactg gttttgatat caaagactat
      601 atttgtaagg atgattacat tgaacttcga atcaatgacc ggcttgctcg tttgtacatc
      661 attccgggaa ttccaaaaga cacaaaattt aacccaaaaa ctagaagaga aattcggaac
      721 attgagtggt tctctattga gaaattgcct tgtcatagaa atgatatgac ccccaaatcc
      781 aaacttggtt tggcacctaa caaatttttt atggccattc cctttatcag accattaagg
      841 gactggcttt ctcgaagatt tggcgattcc tcagacagtg acaatggatt ttcctcaact
      901 ggtagcacgc cggctaaacc cactgtggaa aaattgagtc gaaccaaatt ccgccacagt
      961 cagcagttat ttcctgacgg ttctcctggt gaccagtggg taaagcacag gcaaccactg
     1021 cagcaaaagc catataataa tcattctgaa atgtctgacc ttttaaaagg aaagaatcaa
     1081 agtatgaggg gaaatggcag aaaacagtat caagattcac ctaatcaaaa gaa
//
