LOCUS       sequence-132847-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9
            fusion protein and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pHR-SFFV-KRAB-dCas9-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(95..151)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(191..211)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(218..238)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(251..277)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             complement(257..283)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 actcgtggcc gttcacggag ccctccatgt gcaccttgaa gcgcatgaac tccttgatga
       61 tggccatgtt atcctcctcg cccttgctca ccataggtcc agggttctcc tccacgtctc
      121 cagcctgctt cagcaggctg aagttagtag cggatccgct gctgccgttg ctcccactac
      181 caatgccgtc cactttccgc tttttcttag gatcttccac cttgcgtttt ttcttggggg
      241 agccgctgcc caggctggca taatcgggca cgtcataggg ataggcgtcg cctcccagct
      301 gagacaggtc gatccgtgtc tcgtacaggc cggtgatgct ctggtggatc agggtggcgt
      361 ccagcacctc tttggtgctg gtgtacctct tccggtcgat ggtggtgtca aagtacttga
      421 aggcggcagg ggctcccaga ttggtcaggg taaacaggtg gatgatattc tcggcctgct
      481 ctctgatagg cttgtctctg tgcttgttgt aggcgctcag caccttgtcc agattagcgt
      541 cggccaggat cactctcttg gagaactcgc tgatctgctc gatgatctcg tccaggtagt
      601 gtttgtgctg ttccacaaac agctgtttct gctcattatc ctcgggggag cccttcagct
      661 tctcatagtg gctggccagg tacag
//
