LOCUS       sequence-132848-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9
            fusion protein and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead 
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and 
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029. 
            Epub 2014 Oct 9.
  PUBMED    25307932
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date: 
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
COMMENT     Sequence Label: pHR-SFFV-KRAB-dCas9-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             135..320
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
ORIGIN
        1 ctccgacaga ctgagtcgcc cgggggggat ctggagctct cgagaattct cacgcgtctg
       61 caggatatca agcttgcggt accgcgggcc cgggatccgc caccatggat gctaagtcac
      121 taactgcctg gtcccggaca ctggtgacct tcaaggatgt atttgtggac ttcaccaggg
      181 aggagtggaa gctgctggac actgctcagc agatcgtgta cagaaatgtg atgctggaga
      241 actataagaa cctggtttcc ttgggttatc agcttactaa gccagatgtg atcctccggt
      301 tggagaaggg agaagagccc ggcggttccg gcggagggtc gatggacaag aagtacagca
      361 tcggcctggc catcggcacc aactctgtgg gctgggccgt gatcaccgac gagtacaagg
      421 tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc aagaagaacc
      481 tgatcggcgc cctgctgttc gacagcggag aaacagccga ggccacccgg ctgaagagaa
      541 ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa gagatcttca
      601 gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag tccttcctgg
      661 tggaagagga taagaagcac gagcggcacc
//
