LOCUS       sequence-132849-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of tagRFP targeted to the mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-mitoTagRFP
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(135..863)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             complement(885..959)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     protein_bind    complement(966..990)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttatcgataa gcttgatatc gaattctaga gtcgcggcct cgaatcacca ctttgtacaa
      121 gaaagctggg tttacttgta cagctcgtcc atgccattaa gtttgtgccc cagtttgcta
      181 gggaggtcgc agtatctggc cacagccacc tcgtgctgct cgacgtaggt ctctttgtcg
      241 gcctccttga ttctttccag tctgtggtcc acatagtaga cgccgggcat cttgaggttc
      301 ttagcgggtt tcttggatct gtatgtggtc ttgaagttgc agatcaggtg gcccccgccc
      361 acgagcttca gggccatgtc ggttctgcct tccaggccgc cgtcagcggg gtacagcatc
      421 tcggtgttgg cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgggaag
      481 ttcacccctc tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg
      541 gtagcggtca gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctca
      601 gggaaggact gcttaaagaa atcggggatg ccctgggtgt ggttgatgaa ggttctgctg
      661 ccgtacatga agctggtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc
      721 accttgattc tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac
      781 ttgaagtggt ggttgttcac ggtgccctcc atgtacagct tcatgtgcat gttctcctta
      841 atcagctctt cgcccttaga caccggatcc cccaacgaat ggatcttggc gcgcggcact
      901 gggagccgcc gggccgagcc tgtcaagccc cgcagcagca gcggcgtcag gacggacatg
      961 gtgtcagcct gcttttttgt acaaacttgt gattcgaggg atcccgggcc cgcggtatca
     1021 aatcaattct ttgcc
//
