LOCUS       sequence-132851-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector: Neuroligin 2 shRNA under control of 
            mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Chih B, Engelman H, Scheiffele P
  TITLE     Control of excitatory and inhibitory synapse formation by 
            neuroligins.
  JOURNAL   Science. 2005 Feb 25;307(5713):1324-8. Epub 2005 Jan 27.
  PUBMED    15681343
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2005-02-25"; volume: "307(5713)"; pages: "1324-8. Epub 2005 
            Jan 27"
COMMENT     Sequence Label: pLenLox_U6 Nlgn2
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        80..392
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     311..331
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     448..464
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     449..465
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(507..540)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta
       61 gtaccgggcc cgctctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc
      121 acagacttgt gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt
      181 cctagtaact atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac
      241 tagctacatt ttacatgata ggcttggatt tctataagag atacaaatac taaattatta
      301 ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta aagtaattgt gtgttttgag
      361 actataaata tcccttggag aaaagccttg tttggagcaa gttcaacagc aattcaagag
      421 attgctgttg aacttgctcc ttttttctcg aggtcgacgg tatcgataag ctcgcttcac
      481 gagattccag caggtcgagg gacctaataa cttcgtatag catacattat acgaagttat
      541 attaagggtt ccaagcttaa gcggccgcgt ggataaccgt attaccgcca tcgacattga
      601 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      661 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      721 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      781 tgacgtcaat gggtggagta tt
//
