LOCUS       sequence-132853-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Shcherbakova DM, Verkhusha VV
  TITLE     Near-infrared fluorescent proteins for multicolor in vivo imaging.
  JOURNAL   Nat Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521.
  PUBMED    23770755
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jun 16. doi: 10.1038/nmeth.2521."
COMMENT     Sequence Label: piRFP720-N1
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      31..486
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(118..137)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     328..349
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    493..614
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(504..523)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     558..577
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
       61 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      121 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
      181 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
      241 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
      301 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
      361 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
      421 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
      481 caatttacgc cttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
      541 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      601 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
      661 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
      721 tagagtcgcg gccgcttact cttccatcac gccgatctgc caggccagaa cctg
//
