LOCUS       sequence-132856-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of GFP targeted to the mitochondrial outer 
            membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-eGFPomp25
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(262..972,973..975,976..978))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(262..972,973..975,976..978))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(304..325)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     653..672
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     912..933
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      975..984
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttatcgataa gcttgatatc gaattctaga gtcgcggcct cgagatcctc aaagctgctt
      121 tcggtatctc acgaaggccc aaactgctac catggtgagg gcaaacactg gtagcagcac
      181 catagctaca ggaactccac ttggctctcc ttcacctcga tggaattcga agcttgagct
      241 cgagatctga gtccggccgg acttgtacag ctcgtccatg ccgagagtga tcccggcggc
      301 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc
      361 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      421 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt
      481 gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt
      541 gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      601 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      661 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      721 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta
      781 ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      841 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      901 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      961 ctcgcccttg ctcaccatgg tggcgac
//
