LOCUS       sequence-132865-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of CFP targeted to the mitochondrial matrix.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Mishra P, Carelli V, Manfredi G, Chan DC
  TITLE     Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane
            fusion and couples fusion to oxidative phosphorylation.
  JOURNAL   Cell Metab. 2014 Apr 1;19(4):630-41. doi: 
            10.1016/j.cmet.2014.03.011.
  PUBMED    24703695
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2014.03"; journalName: "Cell Metab"; date: 
            "2014-04-1- 1"; volume: "19"; issue: "4"; pages: "630-41"
COMMENT     Sequence Label: pclbw-mitoCFP
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(121..834,835..837,838..840))
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(166..187)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     515..534
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     774..795
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      837..846
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(874..948)
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc cagaggttga
       61 ttatcgataa gcttgatatc gaattctaga gtcgcggcct cgactagagt cgcggccgct
      121 ttacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
      181 gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta
      241 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
      301 ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttgg ccttgatgcc
      361 gttcttctgc ttgtcggcgg tgatatagac gttgtggctg atgtagttgt actccagctt
      421 gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac
      481 cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa
      541 gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg
      601 cttcatgtgg tcggggtagc ggctgaagca ctgcacgccc caggtcaggg tggtcacgag
      661 ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc
      721 gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc
      781 gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat
      841 ggtggcgacc ggtggatccc ccaacgaatg gatcttggcg cgcggcactg ggagccgccg
      901 ggccgagcct gtcaagcccc gcagcagcag cggcgtcagg acggacatgc tagtcgaggg
      961 atcccgggcc cgcggtatca aatcaattct ttgccaaa
//
