LOCUS       sequence-132880-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Zhang Y, Gao J, Chung KK, Huang H, Dawson VL, Dawson TM
  TITLE     Parkin functions as an E2-dependent ubiquitin- protein ligase and 
            promotes the degradation of the synaptic vesicle-associated protein,
            CDCrel-1.
  JOURNAL   Proc Natl Acad Sci U S A 2000 Nov 21;97(24):13354-9.
  PUBMED    11078524
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2000-11-21"; volume: "97(24)"; pages: 
            "13354-9"
COMMENT     Sequence Label: pRK5-HA-Parkin
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        186..204
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     186..203
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     217..236
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      321..330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             333..359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
       61 ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg cctatagagt ctataggccc
      121 acccccttgg cttcgttaga acgcggctac aattaataca taaccttatg tatcatacac
      181 atacgattta ggtgacacta tagaataaca tccactttgc ctttctctcc acaggtgtcc
      241 actcccaggt ccaactgcac ctcggttcta tcgattgaat tcccgaaccg acagtcggtc
      301 tcttcaccaa ggccattcgc gccaccatgg gctaccccta tgatgtgcct gactacgcag
      361 atctcaatgg tggtggtggt gggtcgacca tgatagtgtt tgtcaggttc aactccagcc
      421 atggtttccc agtggaggtc gattctgaca ccagcatctt ccagctcaag gaggtggttg
      481 ctaagcgaca gggggttccg gctgaccagt tgcgtgtgat tttcgcaggg aaggagctga
      541 ggaatgactg gactgtgcag aattgtgacc tggatcagca gagcattgtt cacattgtgc
      601 agagaccgtg gagaaaaggt caagaaatga atgcaactgg aggcgacgac cccagaaacg
      661 cggcgggagg ctgtgagcgg gagccccaga gcttgactcg ggtggacctc agcagctcag
      721 tcctcccagg agactctgtg gggctggctg tcattctgca cactgacagc aggaaggact
      781 caccaccagc tggaagtcca gcaggtagat caatctacaa cagcttttat gtgtattgca
      841 aaggcccctg tcaaagagtg cagccgggaa aactcagggt acagtgcagc acctgcaggc
      901 aggcaacgct cacc
//
