LOCUS       sequence-132898-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 1 transfer plasmid for pST44 polycistronic plasmid suite; 
            C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as 
            positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc1-DHFRSTR
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             554..577
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(673..691)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      687..734
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attggaattc gctagctcta gaaataattt tgtttaactt taagaaggag atatacatat
       61 gggatccatc agtctgattg cggcgctagc ggtagatcgc gttatcggca tggaaaacgc
      121 catgccatgg aacctgcctg ccgatctcgc ctggtttaaa cgcaacacct taaataaacc
      181 cgtgattatg gggcgccata cctgggaatc aatcggtagg cctttgcccg gccgcaaaaa
      241 tattatcctc agcagtcaac ccgggaccga tgatcgggtt acctgggtta aatcggtcga
      301 cgaagccatc gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg gcggacgcgt
      361 ttatgaacag ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg atgcagaagt
      421 ggaaggcgac acccattttc cggattacga gccggatgac tgggaatcgg tattcagcga
      481 attccacgat gctgatgcgc agaactcgca tagctattgt ttcgaaatcc tcgagcgtcg
      541 tgccggcagc gcttggagcc acccgcagtt cgaaaaataa tgtacaagat ccagatctaa
      601 gcttggtacc acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt
      661 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
      721 tgaggggttt tttgctgaaa ggaggaacta tatcgggatt agcccgccta atgagcgggc
      781 ttttttttaa ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      841 tgagacaata accctgataa atgcttcaat aatattga
//
