LOCUS       sequence-132909-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RGVG-M6 T7 RNA polymerase variant with enhanced 
            transcription of 2' modified RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Meyer AJ, Garry DJ, Hall B, Byrom MM, McDonald HG, Yang X, Yin YW, 
            Ellington AD
  TITLE     Transcription yield of fully 2'-modified RNA can be increased by the
            addition of thermostabilizing mutations to T7 RNA polymerase 
            mutants.
  JOURNAL   Nucleic Acids Res. 2015 Sep 3;43(15):7480-8. doi: 
            10.1093/nar/gkv734. Epub 2015 Jul 24.
  PUBMED    26209133
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv734"; journalName: "Nucleic Acids Res"; date: 
            "2015-09-3- 3"; volume: "43"; issue: "15"; pages: "7480-8"
COMMENT     Sequence Label: pQE-WT-RGVG-M6
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttactggatc tatcaacagg agtccaagct cagctaatta agcttttacg cgaacgcgta
       61 gtccgactct aagatgtcac ggaggttcaa gttaccttta gccggaagtg ctggcatttt
      121 gtccaattga gactcgtgca actggtcagc aatctggtcg tagaaatcag ccagtacatc
      181 acaagactca tatgtgtcaa ccatagtttc gcgcactgct ttgaacaggt tcgcagcgtc
      241 agccggaatg gtaccgaagg agtcgtgaat cagtgcaaaa gattcgattc cgtacttctc
      301 gtgtgcccac actacagtct tacgaaggtg gctaccgtct tggctgccta caaagttagg
      361 agcgatacca gactcctgtt tgtgtgcatc aatctcgcta tctttgttgg tgttaatggt
      421 aggctgtaag cggaactgac cgaggaacat caggttcaag cgcgtctgaa taggcttctt
      481 gtattcctgc cacacaggga aaccatcagg agttacccaa tgcacagcgc aacgcttgcg
      541 aagaatctct ccagtcttct tatctttgac ctcagcagcc agcagcttag cagcagactt
      601 aagccagttc attgcttcaa ccgcagctac caccgtcacg ctcgcagatt cccaaatcag
      661 cttagccatg tatccagcag cctgattcgg ctgagtgaac atcagaccct tgccggaatc
      721 aatagctggc tgaatggtat cttccagcac ttgttgacgg aagccgaact ctttggaccc
      781 cacagccagc gtcatgaccg gacgcttagt cacactgcga gta
//
