LOCUS       sequence-132912-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTET etuAld+Ile1-20-pduD21-224- FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Jakobson CM, Kim EY, Slininger MF, Chien A, Tullman-Ercek D
  TITLE     Localization of Proteins to the 1,2-Propanediol Utilization 
            Microcompartment by Non-native Signal Sequences Is Mediated by a 
            Common Hydrophobic Motif.
  JOURNAL   J Biol Chem. 2015 Aug 17. pii: jbc.M115.651919.
  PUBMED    26283792
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2015 Aug 17. pii: jbc.M115.651919."
COMMENT     Sequence Label: CMJ162
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             666..689
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      721..792
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acttacagtc aatacaggaa attgtaagaa ctcttatagc aaaagcaaag aaaggcagcg
       61 ataaacccgt ctcgtttaat gcgcctgcgg catccacagc accacagacc gctgcgcctg
      121 cgggcgacgg ctttctgacc gaagtgggcg aagcgcgcca gggcactcag caggacgaag
      181 tcattatcgc cgtcggcccg gcatttggcc tggcgcaaac cgtcaatatc gtcggcttac
      241 cgcataagag cattctgcgc gaagtcattg ccggtattga agaagaaggc atcaaggcgc
      301 gcgtgattcg ctgctttaaa tcttccgacg tggcgttcgt cgccgttgaa ggtaaccgcc
      361 tgagcggatc cggcatctcc atcggcatcc agtcgaaagg tactacggtt atccaccagc
      421 aggggctacc gccgctctcc aacctggagc tgttcccgca ggcaccgctg ctgacgctgg
      481 aaacctaccg tcagattggt aaaaacgccg cccgctatgc gaaacgagaa tcaccgcagc
      541 cggtccctac gctcaatgac cagatggcac gcccgaagta ccaggcaaag tcggccattt
      601 tacatattaa agagaccaag tacgtcgtga cgggcaaaaa cccgcaggaa ctgcgcgtgg
      661 cgcttgatta taaagatgac gatgacaagt aaaagctttc gagtaaggat ctccaggcat
      721 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      781 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgg
//
