LOCUS       sequence-132934-         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, 
            Bertrand E
  TITLE     Single mRNA molecules demonstrate probabilistic movement in living 
            mammalian cells.
  JOURNAL   Curr Biol. 2003 Jan 21. 13(2):161-7.
  PUBMED    12546792
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2003 Jan 21. 13(2):161-7."
COMMENT     Sequence Label: pMS2-YFP
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(409..420)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(421..447)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(421..447)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctcgcccttg ctcaccatgg tggcgaccgg tggatccgcg tagatgccgg agtttgctgc
       61 gattgctgag ggaatcgggt ttccatcttt taggagacct tgcattgcct taacaataag
      121 ctcgcagtcg gaattcgtgg cgaaaattgg aatggttagt tccatattta agtacgaacg
      181 ccaggcgcct ttaggcacct cgactttgat ggtgtatttg cgattctgcg cagagctctg
      241 acgaacgcta caggttactt tgtaagcctg tgaacgcgag ttagagctga tccattcagc
      301 gatcccgtta gcgaagttgc ttggggcgac agtcacgtcg ccagttccgc cattgtcgac
      361 gagaacgaac tgagtaaagt tagaagccat tttaacggct agcatatggc ggccttcgat
      421 ggcgtagtcg ggcacgtcgt aggggtagcc catggtggac tagtgatcct gaacggcaga
      481 ggttacggca gtttgtctct cccccttccg ggagccacct tcttctccaa ccgtcccggt
      541 cgcgctctcg gcgcttctga ggagagaact ggctgagtga cgccctttat agattcgccc
      601 ttgtgtcccg ccccttcctt tcccgccctc ccttgcgcta cggggccgcc cgcaccggcc
      661 tacacggagc gcgcgcggcg gagttgttga cgctagggct ccggctccct ggttgggtgt
      721 tctttctgac gcgacaggag gaggagaatg ttcctggtcc tgtcgtcctc ctttcgggtt
      781 tcccgtgcac tcaaaccgag gacttacaga acggaggata aagttaggcc atttttactc
      841 agcttcggag ttcaggctca ttttcagcta aagtctctca ttagtatccc ccc
//
