LOCUS       sequence-132942-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging 
            (the recipient vector can be TetON 3G promoter system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Chen B, Gilbert LA, Cimini BA, Schnitzbauer J, Zhang W, Li GW, Park 
            J, Blackburn EH, Weissman JS, Qi LS, Huang B
  TITLE     Dynamic Imaging of Genomic Loci in Living Human Cells by an 
            Optimized CRISPR/Cas System.
  JOURNAL   Cell. 2013 Dec 19;155(7):1479-91. doi: 10.1016/j.cell.2013.12.001.
  PUBMED    24360272
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.12"; journalName: "Cell"; date: "2013-12-19- 
            19"; volume: "155"; issue: "7"; pages: "1479-91"
COMMENT     Sequence Label: pSLQ1658-dCas9-EGFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..45)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgcccttg ctcacggaac tacctacctt gcgctttttc ttgggagctc cctcatcccc
       61 tccgagctgt gagaggtcaa tccgagtttc gtaaagtccg gtgatgctct ggtggatgag
      121 ggtggcgtcc agcacttctt tggtggaggt atagcgtttc cggtcgatgg tggtgtcgaa
      181 gtacttgaat gcggcagggg caccaagatt ggtgagggtg aacaggtgga tgatattctc
      241 agcctgctcc cgaattggtt tgtctctgtg cttattgtat gcgctaagga ctttatcgag
      301 gttggcatca gcgagaatga cgcgtttaga aaactcggaa atctgctcaa tgatctcgtc
      361 cagataatgc ttatgctgct ccacaaacag ttgcttttgc tcgttgtcct cggggctccc
      421 cttcagcttc tcgtaatggc ttgcaaggta gaggaaattc acgtacttgg aggggagagc
      481 cagctcatta cccttctgaa gttcgccagc ggaggcgagc atccgcttcc gcccattctc
      541 cagttcgaac aggctatact ttgggagctt gatgataagg tccttcttga cttccttgta
      601 acctttggct tcgagaaagt caattgggtt cttctcgaag gagctgcgct ccataatggt
      661 gatacccagc agttccttca cgctcttgag cttcttagac tttccttttt ccaccttagc
      721 cacgaccagg acagaataag ccacagttgg tgaatcgaat ccgccgtact tcttagggtc
      781 ccaatccttc ttccttgcaa tgagcttgtc ggagttgcgc ttggggagga ttgattcctt
      841 agaaaagcct ccgg
//
