LOCUS       sequence-132947-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pFOSGE_CUS1D02
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    67..91
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(620..719)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(736..755)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(737..755)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(760..776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(760..776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
       61 ataatgccaa ctttgtacaa aaaagcaggc ttcatgacca cgttgcgacc cttcacctgc
      121 gacgacctct ttaagttcaa caatgtaaac tttgacccac ttacggagac ctacgggctg
      181 tccttctaca cccagtacct ggccaagtgg ccggagtact ttcagcttgc ggagtcgccc
      241 agcggacaaa taatgggcta catcatgggc aaggtggagg gacacctgga caactggcac
      301 ggacacgtga ctgcgctgac cgtctcgccc gactaccggc gccttggtct ggctgcgctg
      361 ctcatgagtt tcctggagga catttcggag aaaaagcgag catactttgt ggatcttttt
      421 gtgaggaaga gcaaccaggt ggcaataaac atgtacacga accttggcta catcatatac
      481 cgcaccatac ttgagtacta ctccggcgac caggacgagg atgcgtacga catgagaaag
      541 gccttatcca gggacgtcaa caaaaagtcg gtcataccgt acacgcagcc tgtacgactg
      601 gaacatatag atatgaatga cccagctttc ttgtacaaag ttggcattat aagaaagcat
      661 tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat cattatttgc
      721 catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      781 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataa
//
