LOCUS       sequence-132962-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET-L630W
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..42
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(352..370)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(353..370)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(390..407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    396..620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(753..772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             975..987
                     /estimated_length=13
ORIGIN
        1 ganntcggat ctaccntgga ctacaaggac gacgatgaca agaagcctat gtcctatgag
       61 gagaagcggc agctcagctt ggacatcaac aagctccccg gcgagaagtg gggccgcgtg
      121 gtgcacatca tccagtcacg ggagccctcc ctgaagaatt ccaaccccga cgagattgaa
      181 atcgactttg agaccctgaa gccgtccaca ctgcgtgagc tggagcgcta tgtcacctcc
      241 tgtttgcgga agaaaaggaa acctcaagct gagaaagttg atgtgattgc cggctcctcc
      301 aagatgaagg gcttctcgta ataactcgag catgcatcta gagggcccta ttctatagtg
      361 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      421 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      481 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
      541 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
      601 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
      661 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      721 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      781 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt taggnttccg
      841 atttagtgct ttacggcact tcgacccnnn naancttgan nagggtgatg gtncnnnnat
      901 tgnnccntcg ccntgatagn ngnnnttnnn ccnttngnng nnggannnnn ngtnctnnnn
      961 aannggannc ttgtnnnnnn nnnnnnnanc nnnnnacc
//
