LOCUS       sequence-132964-        1252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET-F656W
FEATURES             Location/Qualifiers
     source          1..1252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..43
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(353..371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(391..408)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    397..621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      667..1093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(754..773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     gap             1178..1188
                     /estimated_length=11
     gap             1237..1246
                     /estimated_length=10
ORIGIN
        1 cnagctcggn tctaccatgg actacaagga cgacgatgac aagaagccta tgtcctatga
       61 ggagaagcgg cagctcagct tggacatcaa caagctcccc ggcgagaagc tgggccgcgt
      121 ggtgcacatc atccagtcac gggagccctc cctgaagaat tccaaccccg acgagattga
      181 aatcgactgg gagaccctga agccgtccac actgcgtgag ctggagcgct atgtcacctc
      241 ctgtttgcgg aagaaaagga aacctcaagc tgagaaagtt gatgtgattg ccggctcctc
      301 caagatgaag ggcttctcgt aataactcga gcatgcatct agagggccct attctatagt
      361 gtcacctaaa tgctagagct cgctgattag cctcgactgt gccttctagt tgccagccat
      421 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      481 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      541 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
      601 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
      661 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      721 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      781 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      841 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      901 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      961 atagtggact cttgttccaa ctggaacaac actcaaccct atctcggtct attcttttga
     1021 tttntaaggg attttgccga tttcggccta ttggttaaaa antgagctga tttaccaaaa
     1081 tttaacnnga attaatnnng ggnannnnnn nnnagttagg gntggnaang tcccaggnnc
     1141 nnnnnnnnna annnnnnnaa nnnnnnnncn nnnnnncnnn nnnnnnnngg nannnccnnn
     1201 ngnnncnnnn ggnaanttnn nnanntgnnn nnnnatnnnn nnnnnnatcn nc
//
