LOCUS       sequence-132978-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Position 2 transfer plasmid for pST44 polycistronic plasmid suite; 
            N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV 
            cassette as positive control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Tan S, Kern RC, Selleck W
  TITLE     The pST44 polycistronic expression system for producing protein 
            complexes in Escherichia coli.
  JOURNAL   Protein Expr Purif. 2005 Apr;40(2):385-95.
  PUBMED    15766881
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2005-04"; volume: "40(2)"; pages: "385-95"
COMMENT     Sequence Label: pST50Trc2-CBPNDHFR
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(529..549)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(574..651)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     RBS             665..687
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(742..761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(743..761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagccggat cagcgcgcac gcgtggtacc aagctttgta cattagccgg cacgacgctc
       61 gaggatttcg aaacaatagc tatgcgagtt ctgcgcatca gcatcgtgga attcgctgaa
      121 taccgattcc cagtcatccg gctcgtaatc cggaaaatgg gtgtcgcctt ccacttctgc
      181 atcgatatgc gtcagataca gtttttgcgc ttttggcaag aactgttcat aaacgcgtcc
      241 gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt cgtcgaccga
      301 tttaacccag gtaacccgat catcggtccc gggttgactg ctgaggataa tatttttgcg
      361 gccgggcaaa ggcctaccga ttgattccca ggtatggcgc cccataatca cgggtttatt
      421 taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccatggca tggcgttttc
      481 catgccgata acgcgatcta ccgctagcgc cgcaatcaga ctgatggatc cctggaagta
      541 caggttttca ccaccaccac caccagatcc cagaagtgcc ccggaggatg agattttctt
      601 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tagacatatg
      661 tatatctcct tcttaaagtt aaacaaaatt attgaattcc aattggagct ctccggagcc
      721 ggagggaaac cgttgtggtc tccctatagt gagtcgtatt aatttcgcg
//
